Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1401
8
1
gregorian
2022
11
1
21
8
online
1
fulltext
fa
تأثیر ضدافسردگی اسانس گیاه شاه اسپرغم بر برخی مدلهای تجربی افسردگی
در موش سوری
Antidepressant Activity of Tanacetum Balsamita Essential Oil on Some Experimental Models of Depression in Mice
فارماكولوژي
Pharmacology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">استرس اکسیداتیو نقش مهمی در پاتوفیزیولوژی بسیاری از بیماریها از جمله افسردگی ایفا</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ء</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">می</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">کند. از طرفی مطالعات قبلی، اثر </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">آنتیاکسیدانی گیاه شاه اسپرغم را گزارش نمودهاند. هدف از مطالعه حاضر تعیین اثر ضدافسردگی اسانس شاه اسپرغم در مدلهای حیوانی مدل افسردگی میباشد.<b> </b></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مواد و روشها: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">در مطالعه تجربی حاضر، 84 موش به شکل کاملا تصادفی به 14 گروه </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">۶</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">تایی تقسیم شده و به شکل داخل صفاقی حامل (10</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">میلیلیتر/کیلوگرم</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">)، فلوکستین (20 میلیگرم/کیلوگرم)، ایمیپرامین (30 میلیگرم /کیلوگرم) و اسانس شاه اسپرغم (</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">5/12، 50، 100 و 200 میلیگرم/کیلوگرم) را دریافت نمودند. جهت ارزیابی پتانسیل</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ضدافسردگی از آزمون شنای اجباری و آزمون معلق ماندن دم استفاده شد. فعالیت حرکتی حیوان نیز با آزمون جعبه باز بررسی شد. دادهها با استفاده از آنالیز واریانس یکطرفه و تست تعقیبی </span></span></span>Newman-Kelus<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> تجزیه و تحلیل شدند</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">یافتهها: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">دوزهای 50 تا 100 میلیگرم/کیلوگرم</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">اسانس در هر دو </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">آزمون شنای اجباری و آزمون معلق ماندن دم<b> </b></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">سبب کاهش مدتزمان بیحرکتی میشوند (001/0</span></span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). دوزهای 50 تا 100 میلیگرم/کیلوگرم شاه اسپرغم مدتزمان شناکردن را افزایش دادند (001/0</span></span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). مدتزمان صعود کردن توسط هیچکدام از دوزهای اسانس افزایش معنیداری نیافت (05/0</span></span></span>p><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). همچنین هیچکدام از دوزهای اسانس سبب تغییر معنیدار در تعداد عبور از مربع و روی دوپا ایستادن در آزمون جعبه باز نشدند (05/0</span></span></span>p><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> اسانس شاه اسپرغم</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> از اثر ضدافسردگی در موشهای سوری برخوردار </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">میباشد</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">البته مطالعات بیشتری جهت تعیین مکانیسم عمل آن نیاز میباشد.</span></span></span><i></i></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""> شاه اسپرغم، ضدافسردگی، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">آزمون شنای اجباری، آزمون معلق ماندن دم</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">موش سوری</span></span></span><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Background and Objectives:</span></span></b> <span style="font-size:9.5pt"><span style="line-height:140%">Oxidative stress plays an important role in the pathophysiology of many diseases, including depression. On the other hand, previous studies have reported the antioxidant effect of <i>Tanacetum balsamita</i>. The aim of the present study is to investigate the antidepressant effect of <i>Tanacetum balsamita</i> essential oil (TBEO) in animal models of depression. </span></span><span dir="RTL" lang="AR-SA" style="font-size:9.5pt"><span style="line-height:140%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Materials and Methods: </span></span></b><span style="font-size:9.5pt"><span style="line-height:140%">In this experimental study, 84 mice were randomly divided into 14 groups of 6 and intraperitoneally received vehicle (10 ml/kg), fluoxetine (20 mg/kg), imipramine (30 mg/kg), and TBEO (12.5, 50,100, and 200mg/kg). To evaluate the antidepressant effect, the forced swimming test (FST) and tail suspension test (TST) were used. The animal locomotion was also assessed by open-field test (OFT). Data were analyzed using one-way analysis of variance followed by the Newman-Keuls post hoc test.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Results:</span></span></b> <span lang="EN" style="font-size:9.5pt"><span style="line-height:140%">Doses of 50 to 100 mg/kg of essential oil in both FST and TST caused a significant reduction in the duration of immobility (p<0.001). </span></span><span style="font-size:9.5pt"><span style="line-height:140%">In addition, doses of 50,100 and 200 mg/kg of TBEO significantly increased swimming time (p<0.001). But, climbing time was not significantly increased by all doses of the essential oil (p>0.05). </span></span><span lang="EN" style="font-size:9.5pt"><span style="line-height:140%">Also, none of the essential oil doses caused a</span></span> <span lang="EN" style="font-size:9.5pt"><span style="line-height:140%">significant change in the number of </span></span><span style="font-size:9.5pt"><span style="line-height:140%">crossing and rearing’s in the OFT (p>0.05). </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Conclusion:</span></span></b><i><span style="font-size:9.5pt"><span style="line-height:140%"> Tanacetum balsamita</span></span></i><span style="font-size:9.5pt"><span style="line-height:140%"> essential oil has an anti-depressant effect in mice. Nevertheless, more studies are needed to determine its mechanism of action.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Key</span></span></b><b> </b><b><span style="font-size:9.5pt"><span style="line-height:140%">words:</span></span></b><span style="font-size:9.5pt"><span style="line-height:140%"> <i>Tanacetum balsamita, </i>Antidepressant, Forced swimming test, Tail suspension test,</span></span> <span style="font-size:9.5pt"><span style="line-height:140%">Mice</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><span style="font-size:9.5pt"><span style="line-height:140%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Funding: </span></span></b><span style="font-size:9.5pt"><span style="line-height:140%">This study was funded by Islamic Azad University, Urmia Branch, Iran.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Conflict of interest: </span></span></b><span style="font-size:9.5pt"><span style="line-height:140%">None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Ethical approval: </span></span></b><span style="font-size:9.5pt"><span style="line-height:140%">The Ethics Committee of Islamic Azad University of Urmia Branch approved the study (IR.IAU.URMIA.REC.1399.024).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span dir="RTL" lang="AR-SA" style="font-size:9.5pt"><span style="line-height:140%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:150%">How to cite this article:</span></b><b> </b>Nankali Amir-khosro, Abbasi-Maleki Saeid, Najafi<span style="line-height:150%"> Ghader. Antidepressant Activity of Tanacetum Balsamita Essential Oil on Some Experimental Models of Depression in Mice</span><span lang="EN" style="line-height:150%">. </span><i><span style="line-height:150%">J Rafsanjan</span></i><span style="line-height:150%"> <i>Univ Med Sci</i> 2022; 21 (8): 869-84. [Farsi]</span><b> </b><b><span style="line-height:150%"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
شاه اسپرغم, ضدافسردگی, آزمون شنای اجباری, آزمون معلق ماندن دم, موش سوری
Tanacetum balsamita, Antidepressant, Forced swimming test, Tail suspension test, Mice
869
884
http://journal.rums.ac.ir/browse.php?a_code=A-10-879-1&slc_lang=fa&sid=1
Amir-khosro
Nankali
امیر خسرو
نانکلی
amirkhosro98@gmail.com
6900319475328460051730
6900319475328460051730
No
Islamic Azad University
دانشگاه آزاد اسلامی
Saeid
Abbasi-Maleki2,3
سعید
عباسی ملکی
6900319475328460051731
6900319475328460051731
Yes
Kermanshah University of Medical Science
دانشگاه علوم پزشکی کرمانشاه
Ghader
Najafi
قادر
نجفی
6900319475328460051732
6900319475328460051732
No
Islamic Azad University
دانشگاه آزاد اسلامی