Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.61186/jrums
fa
jalali
1401
8
1
gregorian
2022
11
1
21
8
online
1
fulltext
fa
تأثیر حفاظتی کورکومین و ویتامین D بر تغییرات فیزیولوژیک و هیستوپاتولوژیک بیضه در موشهای صحرایی بالغ تیمار شده با متوترکسات: یک مطالعه تجربی
The Protective Effect of Curcumin and Vitamin D on Physiological and Histopathological Changes of Testis in Adult Rats Treated with Methotrexate: An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">کورکومین و ویتامین </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> دارای فعالیت­ آنتی­اکسیدانی</span></span></span> <span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">می­باشند. هدف از این پژوهش تعیین اثر حفاظتی کورکومین و ویتامین </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> بر تغییرات فیزیولوژیک و هیستوپاتولوژیک بیضه در موش­های صحرایی بالغ تیمار شده با متوترکسات بود. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مواد و روش ها:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> در این مطالعه تجربی، 25 سر موش صحرایی نر بالغ به 5 گروه 5تایی تقسیم شدند. گروه کنترل، گروه شاهد: 1 میلی­لیتر آب مقطر به عنوان حلال، متوترکسات (٥ میلی­گرم/کیلوگرم)، متوترکسات+کورکومین (٢٠٠ میلی</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">گرم/کیلوگرم) + ویتامین</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (500 واحد بین</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">المللی/کیلوگرم)، متوترکسات+کورکومین (4٠٠ میلی­گرم/کیلوگرم) + ویتامین</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (10٠٠ واحد بینالمللی/کیلوگرم). پس از 28 روز تیمار با کورکومین و ویتامین </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> (گاواژ)، خون­گیری مستقیم از قلب جهت اندازهگیری غلظت سرمی هورمون لوتئینی (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">Luteinizing hormone; LH</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">)، هورمون محرک فولیکول (</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">Follicle-stimulating hormone; FSH</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) و هورمون تستوسترون انجام شد. بیضه­ها خارج و تغییرات بافتی بعد از رنگ­آمیزی هماتوکسیلین-ائوزین مورد بررسی قرار گرفت. داده­ها با استفاده از آنالیز واریانس یک­طرفه و آزمون تعقیبی </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">Tukey</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> تجزیه­ و تحلیل شد. </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">یافته­ها:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> سطح هورمون تستوسترون، تعداد اسپرماتوگونی، اسپرماتوسیت، اسپرم و لیدیگ، در گروه تجربی 1 نسبت به گروه­های کنترل و شاهد کاهش معنی­داری نشان داد، اما سطح سرمی هورمون­های </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">LH</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> و </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">FSH</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> افزایش معنی­داری را نشان داد (05/0></span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">p</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). سطح هورمون تستوسترون، تعداد اسپرماتوگونی، اسپرماتوسیت، اسپرم و لیدیگ در گروه­های تجربی 3 و 4 نسبت به گروه تجربی 1، افزایش معنی­داری نشان داد، اما سطح سرمی هورمون­های </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">LH</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> و </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">FSH</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> کاهش معنی­داری نشان داد (05/0></span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">p</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتیجه­گیری:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> احتمالاً کورکومین و ویتامین </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> به دلیل داشتن خاصیت آنتی­اکسیدانی و ضدالتهابی قادر است تغییرات فیزیولوژیک و هیستوپاتولوژیک بیضه در موش­های صحرایی بالغ تیمار شده با متوترکسات را بهبود بخشد.</span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:115%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.5pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">واژه­های کلیدی:</span></span></span></b><span lang="FA" style="font-size:11.5pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""> کورکومین، ویتامین </span></span></span><span dir="LTR" style="font-size:9.5pt"><span style="line-height:107%">D</span></span><span lang="FA" style="font-size:11.5pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">، متوترکسات، تستوسترون، بیضه، موش صحرایی</span></span></span><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span>
<pre style="text-align:justify">
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.5pt"><span style="line-height:140%">Background and Objectives:</span></span></b> <span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Curcumin and vitamin D have antioxidant activit</span></span><span style="font-size:9.5pt"><span style="line-height:140%">ies</span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">. The aim of this study was to determine the protective effect of curcumin and vitamin D on the physiological and histopathological changes of the testis in adult rats treated with methotrexate</span></span><span style="font-size:9.5pt"><span style="line-height:140%">.</span></span><span dir="RTL" lang="AR-SA" style="font-size:9.5pt"><span style="line-height:140%"></span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Materials and Methods:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> In this experimental study, 25 adult male Wistar rats were divided into 5 groups of 5. Control group, sham group: 1 ml of distilled water as a solvent, methotrexate (5 mg/kg), methotrexate (5 mg/kg) + curcumin (200 mg/kg) + vitamin D (500 IU/kg), </span></span><span style="font-size:9.5pt"><span style="line-height:140%">and </span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">methotrexate (5 mg/kg) + curcumin (400 mg/kg) + vitamin D (1000 IU/kg).</span></span> <span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">After 28 days of treatment with curcumin and vitamin D (gavage), blood was taken directly from the heart to measure serum concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH)</span></span><span style="font-size:9.5pt"><span style="line-height:140%">,</span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> and testosterone. The testicles were removed and tissue changes were examined after hematoxylin-eosin staining</span></span><span style="font-size:9.5pt"><span style="line-height:140%">. Data were analyzed using one-way analysis of variance and Tukey's post hoc test.</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Results:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> Testosterone hormone level, number of spermatogonia, spermatocyte, sperm and Leydig showed a significant decrease in </span></span><span style="font-size:9.5pt"><span style="line-height:140%">the </span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">experimental group 1 compared to the control and sham groups, but the serum level of LH and FSH hormones showed a significant increase (p<0.05). The level of testosterone hormone, the number of spermatogonia, spermatocytes, sperm and Leydig in </span></span><span style="font-size:9.5pt"><span style="line-height:140%">the </span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">experimental groups 4 and 3 showed a significant increase compared to </span></span><span style="font-size:9.5pt"><span style="line-height:140%">the </span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">experimental group 1, but the serum level of LH and FSH hormones showed a significant decrease (</span></span><span style="font-size:9.5pt"><span style="line-height:140%">p</span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"><0.05).</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Conclusion:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> Probably, curcumin and vitamin D, due to their antioxidant and anti-inflammatory properties, can improve the physiological and histopathological changes of the testis in </span></span><span style="font-size:9.5pt"><span style="line-height:140%">the </span></span><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">adult rats treated with methotrexate.</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Key words:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> Curcumin, Vitamin D, Methotrexate, Testosterone, Testis, Rat</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"></span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Funding:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> This study did not have any funds.</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Conflict of interest:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> None declared.</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%">Ethical approval:</span></span></b><span lang="X-NONE" style="font-size:9.5pt"><span style="line-height:140%"> The Ethics Committee of Islamic Azad University of Kazerun approved the study (IR.IAU.KAU.REC.1401.030).</span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:140%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span dir="RTL" lang="AR-SA" style="font-size:9.5pt"><span style="line-height:140%"></span></span></b></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:150%">How to cite this article: </span></b><span style="line-height:150%">Dehghani Mahnaz, Shariati Mehrdad, Moghadamnia Davood. The Protective Effect of Curcumin and Vitamin D on Physiological and Histopathological Changes of Testis in Adult Rats Treated with Methotrexate: An Experimental Study.<i> J Rafsanjan</i></span><span style="line-height:150%"> <i>Univ Med Sci</i> 2022; 21 (8): 853-68. [Farsi]</span><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span>
</pre>
کورکومین, ویتامین D, متوترکسات, تستوسترون, بیضه, موش صحرایی
Curcumin, Vitamin D, Methotrexate, Testosterone, Testis, Rat
853
868
http://journal.rums.ac.ir/browse.php?a_code=A-10-5979-1&slc_lang=fa&sid=1
Mahnaz
Dehghani
مهناز
دهقانی
6900319475328460051727
6900319475328460051727
No
Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
دانشگاه آزاد اسلامی، کازرون، ایران
Mehrdad
Shariati
مهرداد
شریعتی
6900319475328460051728
6900319475328460051728
No
Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
دانشگاه آزاد اسلامی، کازرون، ایران
Davood
Moghadamnia
داوود
مقدم نیا
davood.moghadamnia@gmail.com
6900319475328460051729
6900319475328460051729
Yes
Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
دانشگاه آزاد اسلامی، کازرون، ایران