Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.61186/jrums
fa
jalali
1402
2
1
gregorian
2023
5
1
22
2
online
1
fulltext
en
تأثیر عصاره هیدروالکلی میوه گیاه کور بر آنزیمهای کلیدی مسیرهای متابولیسم
گلوکز در سلولهای کبدی موشهای صحرایی دیابتی: یک مطالعه تجربی
The Effect of Hydroalcoholic Extract of Capparis Spinosa Fruit on Key Enzymes of Glucose Metabolism Pathways in the Liver Cells of Diabetic Rats: An Experimental Study
بیوشیمی
Biochemistry
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:99%"><span b="" nazanin="" style="font-family:">چکیده</span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><a name="_Hlk113374001"><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">با توجه به این­که یافتن داروهای جدید با عوارض جانبی کمتر برای کاهش قند در افراد دیابتی ضروری به نظر می­رسد، هدف مطالعه تعیین تأثیر عصاره میوه کور بر آنزیم­های کلیدی مسیرهای متابولیسم گلوکز در سلولهای کبدی موش­های نر دیابتی بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">مواد و روش­ها:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> در این مطالعه تجربی، تعداد 60 سر موش صحرایی به طور تصادفی به 6 گروه تقسیم شدند. گروههای 1 تا 3 به ترتیب حیوانات نرمال که آب مقطر، غلظت 200 و 800 میلیگرم بر کیلوگرم عصاره، و گروههای 4 تا 6 موشهای صحرایی دیابتی که آب مقطر، غلظت200 و 800 میلیگرم بر کیلوگرم عصاره را به ترتیب دریافت کردند. </span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">القاء دیابت با تزریق درون صفاقی ماده استرپتوزوتوسین با دوز 50 میلیگرم بر کیلوگرم صورت گرفت. </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">اندازه­گیری هورمون انسولین و گلوکز سرم با کیت مربوطه، فعالیت آنزیمهای گلوکوکیناز و هگزوکیناز به روش دستی و بیان ژن فسفوفروکتوکیناز </span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">و </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">گلوکز 6 فسفاتاز</span></span></span> <span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">به روش </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%">Real Time-PCR</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> صورت گرفت. دادهها با استفاده از آزمون ناپارامتریک </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%">Kruskal-Wallis</span></span><span style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> و آزمون تعقیبی </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%">Mann-Whitney</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> تجزیه و تحلیل شد.</span></span></span><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%"></span></span></i></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">یافتهها:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> تجویز عصاره، سطح سرمی گلوکز را کاهش و سطح سرمی انسولین و گلوکیناز و میزان بیان ژنهای فسفوفروکتوکیناز-1 را در تمام گروههای دریافت کننده عصاره نسبت به گروههای کنترل افزایش داد و میانگین فعالیت آنزیم هگزوکیناز و بیان ژن </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">گلوکز 6 فسفاتاز</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> در</span></span></span><i> </i><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">بین بیشتر گروه­ها اختلاف معنی­داری نشان داد (001/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:110%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">). </span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">نتیجه­گیری:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> تیمار حیوانات دیابتی با عصاره گیاه کور باعث کاهش معنی­دار قند خون و افزایش میزان انسولین سرمی گردید و هم­چنین منجر به بهبود وضعیت فعالیت آنزیمهای دخیل در گلیکولیز شد. بنابراین، به نظر می­رسد که مصرف این میوه اثرات</span></span></span><i> </i><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">مفیدی بر روی قند خون و ترشح انسولین و هم­چنین متابولیسم قند داشته باشد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:">واژه­های کلیدی:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:110%"><span b="" nazanin="" style="font-family:"> کور، موش صحرایی دیابتی، متابولیسم، گلوکوکیناز، هگزوکیناز </span></span></span><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:110%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:145%">Background and Objectives:</span></span></b></a><span style="font-size:9.0pt"><span style="line-height:145%"> Since finding new drugs with fewer side effects to reduce blood sugar in diabetics seems necessary, the present study aimed to evaluate the effect of the different doses of Capparis spinosa fruit extract on key enzymes of glucose metabolism pathways in the liver cells of diabetic male rats. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Materials and Methods: </span></span></b><span style="font-size:9.0pt"><span style="line-height:145%">In this experimental study, 60 male Wistar rats were randomly divided into 6 groups. Groups 1-3 were normal (nondiabetic) rats that received distilled water, and 200 and 800 mg/kg extract, respectively, and groups 4-6 were diabetic rats that received distilled water, and 200 and 800 mg/kg extract, respectively. Diabetes induced by intraperitoneal injection of 50 mg/kg streptozotocin. Serum levels of insulin and glucose were measured by special kits, the activity of glucokinase and hexokinase was measured manually, and gene expression of phosphofructokinase-1 and glucose-6-phosphatase was determined by Real Time-PCR. Data was analyzed using non-parametric Kruskal-Wallis H test and post hoc Mann-Whitney U test.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:145%"> Administration of Capparis spinosa fruit extract decreased the serum level of glucose but increased the serum level of insulin and glucokinase and the level of expression of phosphofructokinase-1 genes in all groups receiving the extract compared to the control groups, and the average activity of glucose-6-phosphatase and hexokinase in different groups was significantly different (p<0.001).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Conclusion: </span></span></b><span style="font-size:9.0pt"><span style="line-height:145%">The treatment of diabetic animals with Capparis spinosa fruit extract caused a significant decrease in blood sugar and an increase in serum insulin levels, and also led to an improvement in the activity of enzymes involved in glycolysis. Therefore, it seems that the consumption of this fruit has beneficial effects on blood sugar and insulin secretion as well as sugar metabolism</span></span><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:145%">.</span></span><span style="font-size:9.0pt"><span style="line-height:145%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Key words:</span></span></b><span style="font-size:9.0pt"><span style="line-height:145%"> Capparis Spinosa, Diabetic Rats, Metabolism, Glucokinase, Hexokinase</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Funding:</span></span></b><span style="font-size:9.0pt"><span style="line-height:145%"> This study was funded by Rafsanjan University of Medical Sciences.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:145%"> None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">Ethical approval:</span></span></b><span style="font-size:9.0pt"><span style="line-height:145%"> The Ethics Committee of Rafsanjan University of Medical Sciences approved <a>this study.</a></span></span><span style="font-size:8.0pt"><span style="line-height:145%"><span garamond="" style="font-family:"><a class="msocomanchor" href="#_msocom_1" id="_anchor_1" language="JavaScript" name="_msoanchor_1" on<illegal tag>mouseout="msoCommentHide('_com_1')" on<illegal tag>mouseover="msoCommentShow('_anchor_1','_com_1')">[H1]</a> </span></span></span><span style="font-size:9.0pt"><span style="line-height:145%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:145%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="line-height:145%">How to cite this article:</span></span></b><b> </b><span style="font-size:9.0pt"><span style="line-height:145%">Noroozi-Karimabad Mojgan, Nazari Mahmoud, Chahardoli Mehdi, Hajizadeh Mohammad Reza, Mirzaei Mohammad Reza, Assadollahi Zahra, Mahmoodi Mehdi.<b> </b></span></span><span style="font-size:9.0pt"><span style="line-height:145%">The Effect of Hydroalcoholic Extract of Capparis Spinosa Fruit on Key Enzymes of Glucose Metabolism Pathways in the Liver Cells of Diabetic Rats: An Experimental Study</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:145%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:145%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:145%"> <i>Univ Med Sci</i> 2023; 22 (2): 161-74. [Farsi]</span></span> <b><span style="font-size:9.0pt"><span style="line-height:145%"></span></span></b></span></span></span></span></span>
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کور, موش صحرایی دیابتی, متابولیسم, گلوکوکیناز, هگزوکیناز
Capparis Spinosa, Diabetic Rats, Metabolism, Glucokinase, Hexokinase
161
174
http://journal.rums.ac.ir/browse.php?a_code=A-10-89-20&slc_lang=en&sid=1
mojgan
noroozi
مژگان
نوروزی
mojgan.noroozi@yahoo.com
6900319475328460053785
6900319475328460053785
Yes
1. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
مرکز تحقیقات پزشکی مولکولی، پژوهشکده علوم پایه پزشکی،
Mahmoud
Nazari
محمود
نظری
nazary.mahmood@yahoo.com
6900319475328460053786
6900319475328460053786
No
1. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
دانشکده پزشکی
Mehdi
Chahardoli
مهدی
چهاردولی
chahardoli.mahdi@gmail.com
6900319475328460053787
6900319475328460053787
No
1. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
دانشکده پزشکی، دانشگاه آزاد اسلامی، واحد نجفآباد
Mohammad Reza
Hajizadeh
محمدرضا
حاجی زاده
hajizadehus@yahoo.com
6900319475328460053788
6900319475328460053788
No
1. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان،
Mohammad Reza
Mirzaei
محمد رضا
میرزایی
mirzaeemr@gmail.com
6900319475328460053789
6900319475328460053789
No
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان
Zahra
Assadollahi
زهرا
اسداللهی
6900319475328460053790
8373-1503-0002-0000
No
4. Academic Member , MSc , Department of Epidemiology and Biostatistics, Rafsanjan University of Medical Sciences, Rafsanjan, Iran, Phd Student, Department of Epidemiology and Biostatistics, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran
گروه آمار و اپیدمیولوژی، دانشکده بهداشت
Mehdi
Mahmoudi
مهدی
محمودی
ahmoodies@yahoo.com
6900319475328460053791
6900319475328460053791
No
3. Department of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
علوم پزشکی رفسنجان