Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1402
7
1
gregorian
2023
10
1
22
6
online
1
fulltext
fa
اثرات آنتیاکسیدانی سیلیمارین بر اختلالات یادگیری و اضطراب ناشی از اتانول در موش صحرایی: یک مطالعه تجربی
Silymarin Antioxidant Effect on Ethanol-Induced Anxiety and Learning Impairments in Rats: An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><a name="_Hlk113374001"><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b></a><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">اتانول پیامدهای عصبی قوی بر روی مغز دارد که با استرس اکسیداتیو مرتبط است. هدف از این مطالعه تعیین اثر سیلیمارین بر استرس اکسیداتیو، اضطراب و اختلال یادگیری ناشی از مصرف اتانول در موش صحرایی بود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">مواد و روشها:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> در این مطالعه تجربی،<b> </b>35 سر موش صحرایی نر نژاد ویستار با میانگین وزنی 200 گرم به پنج گروه تقسیم شدند. گروه کنترل و چهار گروهی که اتانول 4 گرم بر کیلوگرم را به مدت 14 روز دریافت نمودند. سه گروه تیمار، به ترتیب با دوزهای 50، 100 و 150 میلیگرم بر کیلوگرم </span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">سیلیمارین به مدت 14 روز، درمان شدند. اختلالات شناختی و اضطراب مورد ارزیابی قرار گرفت. میانگین فعالیت آنزیمهای کاتالاز، سوپراکسید دیسموتاز و سطح گلوتاتیون در ناحیه هیپوکامپ اندازه­گیری شد.</span></span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> داده­ها توسط آنالیز واریانس یک­طرفه و آزمون تعقیبی </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">Tukey</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> تجزیه و تحلیل شدند.</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">یافتهها:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> مصرف اتانول شاخص تبعیض (001/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">)، درصد زمان گذرانده شده در بازوی باز (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">%Open Arm Time; %OAT</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) (001/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) و درصد ورود به بازوی باز (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">%Open Arm Entry; %OAE</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) (007/0=</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) را بهطور معناداری نسبت به گروه کنترل کاهش داد. هم­چنین، فعالیت آنزیمهای کاتالاز (001/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">)، سوپراکسیددیسموتاز (002/0=</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) و سطح گلوتاتیون (001/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) نسبت به گروه کنترل کاهش نشان داد. درحالی­که مصرف سیلیمارین با دوز 150 میلی­گرم بر کیلوگرم، </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">OAE</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> (022/0=</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">OAT</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> (023/0=</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) را نسبت به گروه اتانول افزایش داد و هم­چنین فعالیت آنزیمهای کاتالاز (008/0=</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">)، سوپراکسیددیسموتاز (030/0=</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) و سطح گلوتاتیون را (001/0></span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:122%">P</span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">) را نسبت به گروه اتانول افزایش داد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:122%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin"">نتیجهگیری:</span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:122%"><span style="font-family:"B Nazanin""> احتمالاً سیلیمارین با افزایش سطح آنتیاکسیدانی در هیپوکامپ از بروز اختلالات رفتاری ناشی از مصرف اتانول محافظت میکند.</span></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin"">واژههای کلیدی:</span></span></span></b><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""> سیلیمارین، اختلالات شناختی، استرس اکسیداتیو، اضطراب، هیپوکامپ، موش صحرایی</span></span></span>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives:</span></span></b></a><span style="font-size:9.0pt"><span style="line-height:150%"> Ethanol has shown strong neurodegenerative consequences in brain that are associated with oxidative stress. The aim of this study was to investigate the effects of silymarin on oxidative stress, anxiety, and learning disorder induced by ethanol in rats.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> In this experimental study, 35 male Wistar rats with 200gr average weight were divided into five groups. The control group and four groups that received 4 gr/kg ethanol</span></span><b> </b><span style="font-size:9.0pt"><span style="line-height:150%">for 14 days. Three treatment groups were treated with doses of 50, 100, and 150 mg/kg silymarin for 14 days, respectively. Cognitive impairment and anxiety were assessed. The average activity of catalase and superoxide dismutase</span></span> <span style="font-size:9.0pt"><span style="line-height:150%">enzymes and glutathione (GSH) level in the hippocampus were also assessed. Data were analyzed using one-way analysis of variance (ANOVA) and Turkey’s post hoc test.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Ethanol consumption significantly decreased the discrimination index (p<0.001), the percentage of open arm time (%OAT) (p<0.001), and the percentage of open arm entry (%OAE) (p=0.007) compared to the control group. Also, it reduced the activity of antioxidants catalase (p<0.001), superoxide dismutase (p=0.002), and glutathione level (p<0.001) compared to the control group, while silymarin consumption with dose 150 mg/kg significantly increased the discrimination index, OAE (p=0.022), and OAT (p=0.023) compared to the ethanol group. Also, it increased antioxidant activity of catalase (p=0.008), superoxide dismutase (p=0.030), and glutathione (p<0.001) compared to the ethanol group. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Silymarin may protect the hippocampus against behavioral disorder induced by ethanol consumption by increasing the level of antioxidants.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Key words:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Silymarin, Cognitive impairment, Oxidative stress, Anxiety, Hippocampus, Rat</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> This study was</span></span><b> </b><span style="font-size:9.0pt"><span style="line-height:150%">funded by Mazandaran University.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> None declared.</span></span><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical approval:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The Ethics Committee of Mazandaran University approved the study (IR.UMZ.REC.1400.012).</span></span><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span lang="EN" style="font-size:4.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><i><span lang="EN" style="font-size:2.0pt"><span style="line-height:150%"></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="line-height:150%">How to cite this article:</span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Gholizadeh Maryam, Hajizadeh Moghaddam Akbar, Valizadegan Farhad, Khanjani Jelodar Sedigheh Silymarin Antioxidant Effect on Ethanol-Induced Anxiety and Learning Impairments in Rats: An Experimental Study</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:150%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:150%"> <i>Univ Med Sci</i> 2023; 22 (6): 555-66. [Farsi]</span></span> <b><span style="font-size:8.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
سیلیمارین, اختلالات شناختی, استرس اکسیداتیو, اضطراب, هیپوکامپ, موش صحرایی
Silymarin, Cognitive impairment, Oxidative stress, Anxiety, Hippocampus, Rat
555
566
http://journal.rums.ac.ir/browse.php?a_code=A-10-2093-4&slc_lang=fa&sid=1
Maryam
Gholizadeh
مریم
قلی زاده
mry.gh5392@yahoo.com
6900319475328460055140
6900319475328460055140
No
University of Mazandaran
دانشگاه مازندران
Akbar
Hajizadeh Moghaddam
اکبر
حاجی زاده مقدم
a.hajizadeh@umz.ac.ir
6900319475328460055141
6900319475328460055141
Yes
University of Mazandaran
دانشگاه مازندران
Farhad
Valizadehgan
فرهاد
ولی زاده گان
f.valizadegan@umz.ac.ir
6900319475328460055142
6900319475328460055142
No
University of Mazandaran
دانشگاه مازندران
Sedigheh
Khanjani Jelodar
صدیقه
خانجانی جلودار
s.khanjani66@yahoo.com
6900319475328460055143
6900319475328460055143
No
University of Mazandaran
دانشگاه مازندران