Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1402
8
1
gregorian
2023
11
1
22
8
online
1
fulltext
fa
اثر شبه ضداضطرابی عصاره آبی-الکلی کنگر فرنگی (Cynara scolymus L.) در موشهای سوری نر: یک مطالعه تجربی
Anxiolytic-Like Effect of Hydroalcoholic Extract of Cynara Scolymus L. in Male Mice: An Experimental Study
فيزيولوژي
Physiology
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><a name="_Hlk113374001"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">زمینه و هدف: </span></span></span></b></a><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">اضطراب یکی از اختلالات روانی رایج در انسان می­باشد. گیاهان دارویی در درمان اضطراب استفاده می­شوند. هدف از مطالعه حاضر تعیین اثر شبه ضداضطرابی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">کنگر فرنگی (</span></span></span>Cynara scolymus<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">) در موش­های سوری نر بود.</span></span></span> <b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">مواد و روش</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">­</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">ها:</span></span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">در این مطالعه تجربی، تعداد 30 سر موش سوری نر در محدوده وزنی 30-25 گرم به 5 گروه 6تایی شامل گروه 1 (کنترل)، گروه­های 2 تا 4 (دریافت کننده عصاره آبی-الکلی کنگر فرنگی با دوزهای 100، 200 و 400 میلیگرم/کیلوگرم) و گروه 5 (دریافت کننده دیازپام با دوز 2 میلیگرم/کیلوگرم) تقسیم شدند. برای ارزیابی فعالیت ضداضطرابی </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">از آزمون ماز بعلاوه­ای شکل مرتفع (</span></span></span>Elevated Plus-Maze; EPM<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">)</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">30 دقیقه بعد از تزریق داخل صفاقی داروها استفاده شد. محتوای فنولی و فلاونوئیدی تام عصاره توسط روش اسپکتروفتومتری تعیین گردید.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">فعالیت آنتی­اکسیدانی عصاره به­واسطه مهار رادیکال آزاد </span></span></span> 2,2-diphenyl-1-picrylhydrazyl (DPPH)<span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> ارزیابی شد.</span></span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">دادهها توسط آنالیز واریانس یک­طرفه و آزمون تعقیبی </span></span></span>Tukey<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> تجزیه و تحلیل شدند. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">یافته­ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> کنگر فرنگی (200 و 400 میلی­گرم/کیلوگرم) و دیازپام (2 میلیگرم/کیلوگرم) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">به طور </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">معنی­داری منجر به اثر ضداضطرابی در مقایسه با گروه کنترل شدند (05/0</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">></span></span></span>P<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">). </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">محتوای فنولی و فلاونوئیدی تام عصاره</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">به­ترتیب 57/5</span></span></span>±<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">49/59 میلیگرم گالیک اسید/گرم عصاره و 16/17</span></span></span>±<span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">39/93 میلی­گرم روتین/گرم عصاره بود.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> فعالیت مهار رادیکال </span></span></span>DPPH<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> عصاره 03/0</span></span></span>±<span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">32/161 میکروگرم بر میلیلیتر بود.</span></span></span><i></i></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">نتیجه­گیری</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""> نتایج مطالعه حاضر نشان داد که کنگر فرنگی دارای اثر شبه ضداضطرابی<b> </b>می­باشد و به نظر می­رسد بتواند برای کنترل اضطراب استفاده شود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">واژه­های کلیدی: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">اضطراب، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">کنگر فرنگی، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin"">موش سوری</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:135%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-size:1.0pt"><span style="line-height:135%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives:</span></span></b></a><span style="font-size:9.0pt"><span style="line-height:150%"> <a name="_Hlk140739365">Anxiety is one of the common psychological disorders in humans. Medicinal plants are used in the treatment of anxiety. The purpose of the present study was to investigate the anxiolytic-like effect of <i>Cynara scolymus</i> in male mice.</a></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">In this experimental study, 30 male mice weighing 25-30 g were divided into 5 groups of 6 including Group 1 (control), Group 2 to 4 (receiving hydroalcoholic extract of <i>Cynara</i> <i>scolymus</i> with doses of 100, 200, and 400 mg/kg), and Group 5 (receiving diazepam with a dose of 2 mg/kg). To evaluate anxiolytic activity, Elevated Plus-Maze (EPM) test was used<b> </b>30 minutes after intraperitoneal injection of the drugs. Total phenolic and flavonoid content of extract was determined by spectrophotometric method. Also, the antioxidant effect of the extract was assessed using inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. Data were analyzed using one-way analysis of variance and Tukey's post hoc tests.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> <i>Cynara</i> <i>scolymus </i>(200 and 400 mg/kg) and diazepam (2 mg/kg) significantly led to anxiolytic effect<b> </b>compared to the control group (p<b>></b>0.05). Total phenolic and flavonoid content of the extract were 59.49±5.57 mgGAE/g extract and 93.39±17.16 mgRE/g extract, respectively. The DPPH radical scavenging activity of the extract was 161.32±0.03 µg/ml.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The resutls of the present study suggested that <i>Cynara scolymus</i> has anxiolytic-like effect, and it appears that <i>Cynara scolymus</i> can be used to control anxiety.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Key words:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Anxiety,<i> Cynara scolymus</i>, Mice</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">This study was funded by Razi University, Kermanshah, Iran.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical approval: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">The Ethics Committee of Razi University approved the study (IR.RAZI.REC.1401.023).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><i><span lang="EN" style="font-size:2.0pt"><span style="line-height:150%"></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:9.0pt"><span style="line-height:150%">How to cite this article:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Paydar Esmat, Alimohammadi Samad. Anxiolytic-Like Effect of Hydroalcoholic Extract of Cynara Scolymus L. in Male Mice: An Experimental Study</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">. </span></span><i><span style="font-size:9.0pt"><span style="line-height:150%">J Rafsanjan</span></span></i><span style="font-size:9.0pt"><span style="line-height:150%"> <i>Univ Med Sci</i> 2023; 22 (8): 805-16. [Farsi]</span></span> <b><span style="font-size:8.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
اضطراب, کنگر فرنگی, موش سوری
Anxiety, Cynara scolymus, Mice
805
816
http://journal.rums.ac.ir/browse.php?a_code=A-10-4606-3&slc_lang=fa&sid=1
Esmat
Paydar
عصمت
پایدار
paydarsmat@gmail.com
6900319475328460055621
0009-0002-5125-592X
No
Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران.
Samad
Alimohammadi
صمد
علی محمدی
S.alimohammadi@razi.ac.ir
6900319475328460055622
6900319475328460055622
Yes
Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
دانشکده دامپزشکی، دانشگاه رازی،کرمانشاه، ایران.