Journal of Rafsanjan University of Medical Sciences
مجله دانشگاه علوم پزشکی رفسنجان
JRUMS
Medical Sciences
http://journal.rums.ac.ir
69
journal69
1735-3165
2008-7268
10.66224/jrums
fa
jalali
1403
11
1
gregorian
2025
2
1
23
10
online
1
fulltext
fa
سنتز، فرمولاسیون و بررسی ویژگیهای نانوحامل نیوزوم حاوی جینجرول پوششدهی شده با کیتوزان: ارزیابی اثرات ضدمیکروبی، ضدسرطانی و سازگاری زیستی آن:
یک مطالعه آزمایشگاهی
Synthesis, Formulation, and Characterization of Niosome Loaded Gingerol Coated Chitosan: Evaluation of Its Antimicrobial, Anticancer, and Biocompatibility Effects: A Laboratory Study
زيست شناسي
زيست شناسي
پژوهشي
Research
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">زمینه و هدف:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">جینجرول یکی از مهم­ترین ترکیبات فنولی موجود در گیاه زنجبیل می­باشد و هدف­مندسازی انتقال ترکیبات مؤثره گیاهی به سلول از چالش­های مهم محققان می­باشد. هدف از این مطالعه، بارگذاری جینجرول در نانوحامل نیوزوم پوششدهی شده با کیتوزان و تعیین اثرات ضدمیکروبی و ضدسرطانی آن بود. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">مواد و روش­ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> در این مطالعه آزمایشگاهی، ابتدا نانوحامل نیوزوم حاوی جینجرول سنتز شده و به­دنبال آن، نیوزوم­­های سنتز شده با کیتوزان پوشش­دهی شده و خصوصیات ساختاری آن بررسی شد. اثرات ضدمیکروبی و ضدبیوفیلمی نیوزوم­های سنتز شده با استفاده از روش میکرودایلوشن بررسی شد. همچنین، اثرات ضدسرطانی و سازگاری زیستی نیوزوم­های سنتز شده با استفاده از روش رنگ سنجی ارزیابی شد. داده­ها با استفاده از آنالیز واریانس یک­طرفه تجزیه­ و­ تحلیل گردید. </span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">یافته­ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> بررسی ساختار نیوزوم­های حاوی جینجرول پوشش­دهی شده با کیتوزان نشان داد که دارای ساختار کروی بوده و دارای میانگین و انحراف معیار سایز 80/5</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"Cambria",serif">±</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">56/102 نانومتر می­باشد. هم­چنین، نتایج </span></span></span>FTIR<span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> (</span></span></span>Fourier transform infrared spectroscopy<span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">) سنتز نیوزوم­ها را تأیید کرد. نتایج نشان داد که نیوزوم­های سنتز شده اثرات ضدمیکروبی را از 1 تا 4 برابر افزایش داد و در غلظت­های 100 و 200 میکروگرم در میلی­لیتر بقاء سلولی به­ترتیب به­میزان </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">39/1</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"Cambria",serif">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">66/</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">24 و </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">12/1</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"Cambria",serif">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">65/</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">16 درصد کاهش یافت (05/0></span></span></span><b>P</b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">). هم­چنین، نیوزوم­های سنتز شده دارای اثرات سمیت سلولی معناداری علیه رده سلولی </span></span></span>HFF<span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> (</span></span></span>Human<b> </b>foreskin fibroblasts<span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">) نبودند (05/0<</span></span></span><b>P</b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">نتیجه­گیری:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> یافته­های مطالعه حاضر نشان داد که نیوزوم­های سنتز شده می­توانند اثرات ضدمیکروبی، ضدبیوفیلمی و ضدسرطانی جینجرول را افزایش دهند و بنابراین از این نانوحامل می­توان جهت انتقال هدف­مند ترکیبات مؤثره گیاهی استفاده نمود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:120%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><v:roundrect arcsize="0" id="Rounded_x0020_Rectangle_x0020_9" o:gfxdata="UEsDBBQABgAIAAAAIQC75UiUBQEAAB4CAAATAAAAW0NvbnRlbnRfVHlwZXNdLnhtbKSRvU7DMBSF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" style="position:absolute; left:0; text-align:left; margin-left:20px; margin-top:31px; width:467.05pt; height:59.5pt; z-index:-251652608; v-text-anchor:top"> <w:wrap anchorx="margin"> </w:wrap></v:roundrect><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin"">واژه­های کلیدی:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""> نیوزوم، کیتوزان، جینجرول، اثرات ضدمیکروبی، اثرات ضدسرطانی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><a name="_Hlk114425361"><b><span style="font-size:9.0pt"><span style="line-height:150%">Background and Objectives: </span></span></b></a><span style="font-size:9.0pt"><span style="line-height:150%">Gingerol is one of the most important phenolic compounds found in the ginger plant, and targeting the transfer of effective plant compounds to cells is one of the important challenges of researchers. The aim of this study was to load gingerol in niosome nanocarrier coated with chitosan and determine its antimicrobial and anticancer effects. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Materials and Methods:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> In this laboratory study, niosome nanocarrier containing gingerol was synthesized and then, synthesized niosomes were coated with chitosan and its structural properties were investigated. The antimicrobial and anti-biofilm effects of the synthesized niosomes were investigated using the microdilution method. Also, anticancer effects and biocompatibility of synthesized niosomes were evaluated using colorimetric method. Data were analyzed using one-way ANOVA.</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Results:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Examining the structure of niosomes containing gingerol coated with chitosan showed that it has a spherical structure and an average size of 102.56±5.80 nm. Also, the results of Fourier transform infrared spectroscopy (FTIR) confirmed the synthesis of niosomes. The results showed that the synthesized niosomes increased the antimicrobial effects 1 to 4 times, and at concentrations of 100 and 200 µg/ml, cell survival was decreased 24.66±1.39% and 16.65±1.12%, respectively (p<0.05). Also, the synthesized niosomes did not have any significant cytotoxic effects against the human foreskin fibroblasts (HFF) cell line (p>0.05).</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conclusion</span></span></b><b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> The results showed that the synthesized niosomes can increase the antimicrobial, anti-biofilm, and anti-cancer effects of gingerol, and therefore this nanocarrier can be used for targeted delivery of effective herbal compounds.</span></span><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">Key words:</span></span></b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%"> Niosome, Chitosan, Gingerol, Antimicrobial effects, Anticancer effects</span></span><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%"></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Funding</span></span></b><b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">:</span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">This study was done at personal expense.</span></span><b><span style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Conflict of interest:</span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">None declared.</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Ethical considerations</span></span></b><b><span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">:</span></span></b> <span lang="EN" style="font-size:9.0pt"><span style="line-height:150%">The Ethics Committee of Islamic Aazd University approved the study (IR.IAU.REC.1402.129).</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">Authors’ contributions:</span></span></b><b><span dir="RTL" lang="FA" style="font-size:9.0pt"><span style="line-height:150%"></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Conceptualization:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Methodology:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Data collection:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Formal analysis:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Supervision:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Project administration: </span></span></b><span style="font-size:9.0pt"><span style="line-height:150%">Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Writing – original draft:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:150%"><span style="tab-stops:-45.0pt"><span style="text-autospace:none"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:9.0pt"><span style="line-height:150%">- Writing – review & editing:</span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"> Amir Mirzaie</span></span></span></span></span></span></span>
نیوزوم, کیتوزان, جینجرول, اثرات ضدمیکروبی, اثرات ضدسرطانی
Niosome, Chitosan, Gingerol, Antimicrobial effects, Anticancer effects
887
903
http://journal.rums.ac.ir/browse.php?a_code=A-10-4306-4&slc_lang=fa&sid=1
Amir
Mirzaie
امیر
میرزایی
Amir_mirzaie92@yahoo.com
6900319475328460059616
6900319475328460059616
Yes
Islamic Azad University
دانشکده علوم پایه، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران