Journal of Rafsanjan University of Medical Sciences

  Background and Objectives: Bacterial meningitis especially meningococcal meningitis is a serious and sometimes fatal infection . The aim of this study was the determination of meningitis disease by routine and molecular assay s .

  Materials and Methods : CSF samples were collected from 275 children and agglutination, bacteriological tests were achieved. DNA extraction was done according to phenol-chlorophorm method and PCR was carried out by the specific primers.

  Results : The mean age of the patients was 42±2 month. Younges was 11 days and the oldest child was 11 years old. Meningococcal meningitis was more common in the male gender. Totally, Neisseria meningitidis was found in 3 (1.1%), 5(1.8%) and 10(3.6%) by culture, agglutination and PCR assays, respectively.

  Conclusion : One of the most common etiological agents of meningitis is Neisseria meningitidis in the Azerbaijan state. Molecular diagnosis has higher sensitivity and specifity in comparison to culture and agglutination tests.

  Key words : Meningitis, Neisseria meningitidis , PCR

 Funding: This research was funded by Tropical and Infectious Diseases Research Center of Tabriz University of Medical Sciences.

 Conflict of interest: None declared.

 Ethical approval: The committee of the Research Council and ethical committee of Tabriz University of Medical Sciences was approved the study.

  How to cite this article : Ghotaslou R, Farajnia S. Detection of Neisseria meningitidis in Pediatrics Meningitis by PCR, in Comparison to Culture and Agglutination Tests (Short Report). J Rafsanjan Univ Med Scie 2012 11(6): 605-610. [Farsi]