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URL: http://journal.rums.ac.ir/article-1-1682-en.html
Influence of Nitric Oxide Synthase Inhibitor in the Nucleus Accumbens on Improving Effect of Nicotine in Ethanol-Induced Amnesia
M. Piri[1], A. Moshfegh[2], N. Raoufi2, MS. Shahin[3]
Received: 15/04/2013 Sent for Revision: 12/05/2013 Received Revised Manuscript: 01/07/2014 Accepted: 07/07/2014
Background and Objective: Nitric oxide mediated some behavioral effects of nicotine and ethanol in the nucleus accumbens. In the present study, the effects of L-NAME (nitric oxide synthase inhibitor) in the nucleus accumbens on nicotine’s effect in ethanol-induced amnesia were investigated.
Materials and Methods: In this experimental study 96 adult male wistar rats were used (12 groups). It was used inhibitory avoidable memory step-through model in order to assess memory. The step-through latency for the assessment of memory measured in male Wistar rats. Cannula were bilaterally implanted in the NAC shell of all animals. One-way analysis of variance (ANOVA) followed by multiple comparisons of Tukey test, were used for data analysis. p<0.05 was statistically considered significant difference among groups.
Results: Ethanol induced amnesia (p<0.001) and state-dependent learning (p<0.001). Nicotine injection (p<0.001) or L-NAME (p<0.010) before the test prevented amnesia. Co-administration of an ineffective dose of nicotine with L-NAME potentiated improving effect of L-NAME on memory impaired by ethanol (p<0.001). On the other hand, pre-test intra-NAc injection of L-NAME impairs inhibitory avoidable memory by itself (p<0.001).
Conclusion: The results indicated that L-NAME in the nucleus accumbens potentiated improving effect of nicotine on the ethanol-induced amnesia.
Key words: Nitric oxide synthase, Nicotine, Ethanol amnesia, Nucleus accumbens, Rat
Funding: This research was funded by Lahijan Branch of Islamic Azad University.
Conflict interest: None declared.
Ethical approval: The Ethics Committee of Tehran University of Medical Sciences approved the study.
How to cite this article: Piri M, Moshfegh A, Raoufi N, Shahin MS. Influence of Nitric Oxide Synthase Inhibitor in the Nucleus Accumbens on Improving Effect of Nicotine in Ethanol-Induced Amnesia. J RafsanjanUniv Med Sci 2014; 13(5): 435-44. [Farsi]
[1]- Assistant Prof., Dept. of Biology, Islamic Azad University, Ardabil branch, Ardabil, Iran
(Corresponding Author) Tel: (0451) 7728026, Fax: (021) 554224641, E-mail: biopiri@yahoo.com
[2]- Assistant Prof., Dept. of Biology, Islamic Azad University, Lahijan Branch, Lahijan, Iran
[3]- MSc of Biology, Islamic Azad University, Shahr-e-rey branch, Tehran, Iran
Received: 2013/04/9 | Accepted: 2013/09/30 | Published: 2014/11/25
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