BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
URL: http://journal.rums.ac.ir/article-1-2296-en.html
Effect of Aloe Vera Hydroalcoholic Extract on Blood Glucose, Serum Insulin and the Key Enzymes in Metabolic Pathways of Glycolysis and Gluconeogenesis in Hepatocytes of Type 1 Diabetic Rats
M. Chahardoli[1], M. Mahmoodi[2], M.R. Hajizadeh[3], H. Khoramdel Azad[4], A.R. Khoshdel3, M.R. Mirzae[5]
Received: 16/06/2014 Sent for Revision: 26/07/2014 Received Revised Manuscript: 04/09/2014 Accepted: 21/09/2014
Background and Objective: Recently, it is considered the role of hypoglycemic agents specially medicinal plants derivatives in treatment of diabetes mellitus. Aloe Vera contains phenolic compounds and flavonoids with antioxidant properties that reduces the complications of the disease.The aim of study was to investigate the effects of hydroalcoholic extract of Aloe Vera in serum levels of glucose and insulin and key enzymes in metabolic pathways of glycolysis and gluconeogenesis in streptozotocin-induced diabetic Wistar rats.
Materials and Methods: A total of 50 rats (initial weight of 220 ±10 g) were divided into five groups. Two months after treatment with Aloe Vera extract, the rats were sacrificed and blood glucose and serum insulin levels were measured. It was also isolated the liver then, extracted mRNA from it, expression levels of Glucokinase and Phosphoenol pyruvate carboxy kinase enzymes were measured by Real Time PCR.
Results: The results showed that the extract of Aloe Vera at doses of 100, 200 and 400 mg/kg increase insulin secretion (p<0.05), hypoglycemia (p<0.001), increasing expression of Glucokinase enzyme (p<0.05) and reducing expression of the Phosphoenol pyruvate carboxy kinase enzyme (p<0.05) in diabetic rats.
Conclusion: Not only dose Aloe Vera hydroalcoholic extract influence on insulin secretion, it also affects on key enzymes gene expression of Glycolysis and Gluconeogenesis pathways. Hence, this extract can be effective for improvement of diabetic complications.
Key words: Glycolysis and Gluconeogenesis pathways key enzymes, Streptozotocin, Aloe Vera, Rat
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.
How to cite this article: Chahardoli M, Mahmoodi M, Hajizadeh MR, Khoramdel Azad H, Khoshdel AR, Mirzae MR. Effect of Aloe Vera Hydroalcoholic Extract on Blood Glucose, Serum Insulin and the Key Enzymes in Metabolic Pathways of Glycolysis and Gluconeogenesis in Hepatocytes of Type 1 Diabetic Rats. J RafsanjanUniv Med Sci 2014; 13(8): 668-82 [Farsi]
[1]- MSc Student of Clinical Biochemistry, Dept. of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
[2]- Prof., of Clinical Biochemistry, Molecular Medicine Research Center and Dept. of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
(Corresponding Author) Tel: (034) 34339660, Fax: (034) 34339660, E-mail: Mahmoodies@yahoo.com
[3]- Assistant Prof., of Clinical Biochemistry, Molecular Medicine Research Center and Dept. of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
[4]- MSc in Immunology Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
[5]- Assistant Prof., of Genetics, Molecular Medicine Research Center and Dept. of Genetics, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Received: 2014/09/9 | Accepted: 2015/01/19 | Published: 2015/01/19
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
