Volume 4, Issue 3 (6-2005)                   JRUMS 2005, 4(3): 152-157 | Back to browse issues page

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Fathi-Moghaddam H, Mohammadpour Kargar H, Kesmati M. Effects of Paragigantocellularis Lateralis (LPGi) Nucleus Electrical Lesion on Withdrawal Syndrome in Morphine Dependent Male Rats. JRUMS. 2005; 4 (3) :152-157
URL: http://journal.rums.ac.ir/article-1-24-en.html
استاديار گروه آموزشي فيزيولوژي، دانشگاه علوم پزشكي جندي‌شاپور، اهواز
Abstract:   (11788 Views)
Effects of Paragigantocellularis Lateralis (LPGi) Nucleus Electrical Lesion on Withdrawal Syndrome in Morphine Dependent Male Rats


  H. Fathi-Moghaddam PhD [1] , H. Mohammadpour Kargar MSc [2] , M. Kesmati PhD [3]


  Background and Objective: Nucleus paragigantocellularis lateralis (LPGi) involves in several physiological functions such as cardiovascular regulation, sexual behavior and pain. There is little evidence about its role on opiate withdrawal. In this research the effect of LPGi electrical lesion on withdrawal syndrome in morphine-dependent rats was studied.

  Materials and Methods: N-MRI rats were divided into three groups: Control, Sham and Lesion group. Animals were anaesthetized using ketamine (110 mg/kg) and rampone (3 mg/kg) mixture injection (i.p). Stainless steel electrodes were placed in LPGi (AP=11.8, L=±1.6, DP=10.5) bilaterally. LPGi was lesioned using electrical current (1 mA, DC) for 6 seconds.

  After 10 days recovery period animals were addicted by morphine injection two times a day for 4 days (every day 25, 30, 35, 40 mg/kg respectively). In the fifth day 40mg/kg morphine have been injected to animals and after 30 min withdrawal syndrome was induced by naloxane injection (4 mg/kg i.p) and evaluated withdrawal sign (rearing, ptosis of eye lid, teeth chattering, grooming, wet dog shakes, paw tremor, body tremor, number of ejaculation, defecating, jumping).

  Result: Our results showed that LPGi lesion only can decreased ejuculation, paw tremor and wet dog shakes significantly (p<0.02) but LPGi lesion did not affect other withdrawal signs.

  Conclusions: Our data showed that LPGi altered only ejaculation, paw tremor and wet dog shakes. It seems that LPGi have critical role in those sings and other withdrawal sing related to other nucleus such as PGi and LC.


  Key words: Electrical Lesion, Lateral Paragigantocellularis, Withdrawal Syndrome, Rat, Morphine, Addiction


  [1] - ( Corresponding author ) Assistant Professor, Dept. of Physiology, Faculty of Medicine, Jondishapour University of Medical
Sciences, Ahwaz

  Tel: ( 0611)4437381 , Fax:(0611)3332036, E-mail: hfmoghaddam@yahoo.com

  [2] - Academic Member, Dept. of Biology, Islamic Azad University, Damghan

  [3] - Assistant Professor, Dept. of Biology, Shahid Chamran University, Ahwaz

Full-Text [PDF 451 kb]   (1675 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2005/12/3

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