The Effect of Ferulic Acid on Cognitive Disorder And Brain Structural Deficit in Mice in Cuprizone Induced Animal Model of Multiple Sclerosis
M. Ghobadi[1], B. Arji roodsari[2], M. Yadegari[3], F. Homayouni-Moghadam[4], M.E. Rezvani[5]
Received: 03/05/2017 Sent for Revision: 07/06/2017 Received Revised Manuscript: 05/07/2017 Accepted: 08/07/2017
Background and Objective: Ferulic acid is a polyphenolic compound derived from some fruit and different cereals. Previous studies have shown that this compound has antioxidant, anti-apoptosis, and differentiation properties in the nervous system. The aim of this study was to evaluate the effect of ferulic acid on the death of oligodendrocytes and demyelination in male C57B6 / J mice in cuprizone induced animal model of multiple sclerosis.
Material and methods: In this study
, demyelination was induced by oral administration of rats with the 0.2% cuprizone that was added to the usual diet during 6 weeks. Animals were fed daily with ferulic acid at doses of 10or 100 mg / kg of body weight. At the end of the sixth week, the passive avoidance and Y maze test were performed. Then, animal brains were removed and fixed to histological studies using Luxol–Fast-Blue staining
Results: The results of this study showed that ferulic acid reduced the random entry of animals into the arms of the Y maze in compared to the cuprizone treated group [p<0.01], but the sequential entry of animals into the Y maze were significantly increased [P<0.05]. A significant improvement in passive avoidance test was observed in the groups treated with ferulic acid at dose of 10 [p<0.05]. Histological evaluations showed the lower demyelination in Luxol-Fast-Blue in the group treated with ferulic acid.
Conclusion: The results of this study showed that ferulic acid plays a neuroprotective role in cuprizone models of multiple sclerosis through reducing neuronal demyelination and oligodendrocytes death.
Key words: Ferulic acid, Demyelination, Memory, Cuprizone, Mice
Funding: This research was funded by Shahid Sadoughi University of Medical Sciences
Conflict of interest: None declared.
Ethical approval: The Ethical Committee of Shahid Sadoughi University of Medical Sciences approved the study.
How to cite this article: Ghobadi M, Arji roodsari B, Yadegari M, Homayouni-Moghadam F, Rezvani M.E. The Effect of Ferulic Acid on Cognitive Disorder And Brain Structural Deficit in Mice in Cuprizone Induced Animal Model of Multiple Sclerosis.
J Rafsanjan Univ Med Sci 2017; 16(6): 541-52. [Farsi]
[1]- MSc in Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
[2]- MSc in Anatomy, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
[3]- PhD in Anatomy, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
[4]- PhD in Physiology, Dept. of Cell Biotechnology, Cell Sciences Research Center, Royan Institute, Isfahan, Iran
[5]- PhD in Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
(Corresponding Author) Tel: (035)38203411, Fax: (035)38203411, E-mail: erezvani@yahoo.com