Journal of Rafsanjan University of Medical Sciences

Diagnosis of Bacterial Infections; Traditional and Molecular Methods:
A Narrative Study
 
A[j1] . Darekordi[1], E. Rezazadeh Zarandi[2], S. Assar[3], O. Rezahosseini[4], Sh. Assar[5]
 
 
 
 
 
 
Received: 28/04/2018  Sent for Revision: 17/07/2018    Received Revised Manuscript: 24/09/2018              Accepted: 08/10/2018
 
 
Background and Objectives: Traditionally, diagnosis of the agents of infectious diseases is based on conventional or phenotypic methods. Due to the lack of growth of some infectious agents, these methods are very difficult or impossible for diagnosis of some bacteria. The advent of molecular techniques has more or less influences on the detection of microbial infections. This article addresses the advantages and disadvantages of diagnostic molecular and conventional methods in detecting pathogenic microorganisms. Traditional diagnostic methods such as phenotypic, chemical and serologic characteristics are still used for detection of infectious diseases. In addition to diagnosing the bacterial agents, varient molecular methods may also detect virulence factors and antimicrobial resistance genes at a higher rate, sensitivity and accuracy. It seems that the advanced molecular methods can reduce diagnosis time, mortality rate and costs of laboratory diagnosis, and shorten the duration of hospitalization.
Key words: Diagnosis, Infectious diseases, Traditional, Molecular, Method
 
Funding: This study did not have any funds.
Ethical approval: None declared.
Conflict of interest: None declared.
 
 
How to cite this article: Darekordi A, Rezazadeh Zarandi E, Assar S, Rezahosseini R, Assar Sh. Diagnosis of Bacterial Infections; Traditional and Molecular Methods: A Narrative Study. J Rafsanjan Univ Med Sci 2018; 17 (9): 865-80. [Farsi]