Anti-biofilm [j1] Activity of Synthesized Silver Nanoparticles Using Asphodelus dendroides Extract against Antibiotic Resistant and Biofilm Forming Klebsiella pneumoniae Clinical Strains: A Laboratory Study
M. Sabahi[1], S. M. M. Hamdi[2], A. Mirzaie[3]
Received: 30/06/2019 Sent for Revision: 30/07/2019 Received Revised Manuscript: 09/11/2019 Accepted: 30/11/2019
Background and Objectives: Synthesis of silver nanoparticles (AgNPs) using plant extract is cost effective, while, one of the applications of AgNPs is its antimicrobial and anti-biofilm activities. The aim of this study was to investigate the anti-biofilm activity of synthesized AgNPs using A. dendroides against antibiotic resistant and biofilm forming clinical isolates of Klebsiella pneumoniae.
Materials and Methods: In this laboratory study, the AgNPs were first synthesized using
Asphodelus dendroides extract and their size and structural characteristics were determined using various physico-chemical methods. Subsequently, the antimicrobial and anti-biofilm activities of synthesized AgNPs on antibiotic resistant and biofilm forming clinical isolates of
K. pneumoniae were investigated via minimum inhibitory concentration (MIC) and Real Time PCR, respectively.
Results: The results of the UV-vis test showed that the synthesized AgNPs had a maximum absorbance at 420 nm. The SEM (scanning electron microscope), TEM (transmission electron microscopy) and XRD (X-ray powder diffraction) results showed that the synthesized AgNPs had a spherical structure with a mean size of 30.41±22 nm. Also, AgNPs had significant antimicrobial and anti-biofilm activities against
K. pneumoniae strains, so that the AgNPs down-regulated the expression of biofilm coding gene (
mrkA) in
K. pneumonia strains (p<0.001).
Conclusion: The results of this study showed the antimicrobial and anti-biofilm activities of AgNPs against K. pneumoniae strains. According to the results, with further studies, the synthesized AgNPs may be used as biofilm inhibitors in future.
Key words: Silver nanoparticle,
Klebsiella pneumoniae, Antibacterial activity
, Anti-biofilm activity,
mrkA gene
Funding: This research was funded by Islamic Azad University, Central Tehran Branch.
Conflict of interest: None declared.
Ethical approval: The Ethical Committee of Islamic Azad University of Central Branch approved the study (IR.IAU, Tehran.REC.1397.019)
How to cite this article: Sabahi M, Hamdi S M M, Mirzaie A
. Anti-biofilm Activity of Synthesized Silver Nanoparticles Using
Asphodelus dendroides Extract Against Antibiotic Resistant and Biofilm Forming
Klebsiella pneumoniae Clinical Strains: A Laboratory Study.
J Rafsanjan Univ Med Sci 2020; 18 (12): 1233-52 [Farsi]
[1]- MSc, Dept. of Biology, Islamic Azad University, Central Tehran Branch, Tehran, Iran, ORCID: 0000-0002-8214-6315
[2]- Associate Prof., Dept. of Biology, Islamic Azad University, Central Tehran Branch, Tehran, Iran, ORCID: 0000-0001-7167-1352
(Corresponding Author): Tel: (021) 88074907, Fax: (021) 88074907, E-mail: m.hamdi@iauctb.ac.ir
[3]- Assistant Prof., Dept. of Biology, Islamic Azad University, Roudehen Branch, Roudehen, Iran, ORCID: 0000-0002-0941-7828