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JRUMS 2026, 25(1): 51-62 |
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Ethics code: IR. IAU. TNB. REC. 1403.135
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Siasi E, Daneshvar E, Takbiri L. Study of nheA and cytK Genes Frequency in Bacillus Cereus Isolated from Fresh Rainbow Trout Supplied in Fish Distribution Centers in Tehran: A Laboratory Study. JRUMS 2026; 25 (1) :51-62
URL: http://journal.rums.ac.ir/article-1-7878-en.html
URL: http://journal.rums.ac.ir/article-1-7878-en.html
Department of Microbiology, School of science, NT.C., Islamic Azad University, Tehran, Iran.
Abstract: (5 Views)
Background and Objectives: Seafood has a very high nutritional value and can have effect on social health. Bacillus cereus is one of the most important pathogens of seafood poisoning. This study was aimed to study nheA and cytK genes frequency in Bacillus cereus isolated from fresh rainbow trout.
Materials and Methods: In this in vitro study, thirty samples of fresh rainbow trout, supplied in fish distribution centers in Tehran, were randomly collected in 2024, and transferred to the laboratory. The samples were cultured in BHI (Brain heart infusion) agar medium. Then colonies were cultured in MYP (Mannitol egg Yolk Polymyxin) agar-specific medium, and Gram staining, catalase testing, and PCR targeting the 16S rRNA gene were performed to isolate and identify Bacillus cereus. Sequencing of the 16S rRNA gene, BLAST (Basic Local Alignment Search Tool) analysis using the National Center for Biotechnology Information (NCBI) database, and phylogenetic analysis using MEGA 6.0 were performed, and virulence genes (nheA and cytK) were identified by PCR in the isolated Bacillus cereus bacteria. Data was analyzed by t-test.
Results: From 30 studied samples, 2 samples (6.66%) of Bacillus cereus were isolated and identified, and 100% of them carried nheA and cytK pathogen genes.
Conclusion: The present study indicated that preventing contamination of seafood with Bacillus cereus is essential, as all isolated strains carried virulence genes. Further research on this topic is recommended.
Keywords: Bacillus cereus, nheA and cytK genes, Rainbow trout
Funding: This study did not have any funds.
Ethical approval: The Ethics Committee of NT.C., Islamic Azad University, Tehran Branch, approved the study (IR.IAU.TNB.REC.1403.135).
Conflict of interest: None declared.
Authors’ contributions:
- Conceptualization: Elham Siasi
- Methodology: Elahe Daneshvar
- Data collection: Elahe Daneshvar
- Formal analysis: Elham Siasi
- Supervision: Laya Takbiri
- Project administration: Elham Siasi
- Writing – original draft: Elham Siasi
- Writing – review & editing: Elham Siasi, Laya Takbiri
Materials and Methods: In this in vitro study, thirty samples of fresh rainbow trout, supplied in fish distribution centers in Tehran, were randomly collected in 2024, and transferred to the laboratory. The samples were cultured in BHI (Brain heart infusion) agar medium. Then colonies were cultured in MYP (Mannitol egg Yolk Polymyxin) agar-specific medium, and Gram staining, catalase testing, and PCR targeting the 16S rRNA gene were performed to isolate and identify Bacillus cereus. Sequencing of the 16S rRNA gene, BLAST (Basic Local Alignment Search Tool) analysis using the National Center for Biotechnology Information (NCBI) database, and phylogenetic analysis using MEGA 6.0 were performed, and virulence genes (nheA and cytK) were identified by PCR in the isolated Bacillus cereus bacteria. Data was analyzed by t-test.
Results: From 30 studied samples, 2 samples (6.66%) of Bacillus cereus were isolated and identified, and 100% of them carried nheA and cytK pathogen genes.
Conclusion: The present study indicated that preventing contamination of seafood with Bacillus cereus is essential, as all isolated strains carried virulence genes. Further research on this topic is recommended.
Keywords: Bacillus cereus, nheA and cytK genes, Rainbow trout
Funding: This study did not have any funds.
Ethical approval: The Ethics Committee of NT.C., Islamic Azad University, Tehran Branch, approved the study (IR.IAU.TNB.REC.1403.135).
Conflict of interest: None declared.
Authors’ contributions:
- Conceptualization: Elham Siasi
- Methodology: Elahe Daneshvar
- Data collection: Elahe Daneshvar
- Formal analysis: Elham Siasi
- Supervision: Laya Takbiri
- Project administration: Elham Siasi
- Writing – original draft: Elham Siasi
- Writing – review & editing: Elham Siasi, Laya Takbiri
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Citation: Siasi E, Daneshvar E, Takbiri L. Study of nheA and cytK Genes Frequency in Bacillus Cereus Isolated from Fresh Rainbow Trout Supplied in Fish Distribution Centers in Tehran: A Laboratory Study. J Rafsanjan Univ Med Sci 2026 Apr; 25 (1): 51-62. doi: 1066224/jrums.25.1.51 [Farsi]
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Type of Study: Research |
Subject:
Microbiology
Received: 2025/11/2 | Accepted: 2026/05/23 | Published: 2026/06/20
Received: 2025/11/2 | Accepted: 2026/05/23 | Published: 2026/06/20
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