Showing 3 results for Hesam
A. Hesampour, N. Mohandesi,
Volume 17, Issue 6 (10-2018)
Abstract
Background and Objectives: Laccase is the most abundant member of protein family that catalyzes the oxidation of substituted phenols. Laccases are used as biocatalysts for decolorization and bleaching in dye industries, detoxification in environment, and juice clarification in food industries. The present study aimed at producing recombinant laccase, purifying with high yield and fold, and characterizing biochemical and kinetic properties of the purified laccase.
Materials and Methods: In this laboratory study, recombinant Trametes laccase, which was successfully expressed in yeast host in extracellular form, was purified through ultrafiltration and gel chromatography methods. The purification process yield and fold was determined and after molecular weight determination, physiochemical properties of recombinant laccase were studied and its thermostability was determined. All tests were conducted with at least 3 times repetition and mean and standard deviation calculation.
Results: Purification strategy showed that laccase was successfully purified with 5.67 fold and 49.7% yield and the molecular weight of recombinant laccase was determined 65KDa. Optimum pH and temperature of the recombinant laccase were determined 4.8 and 60°C, respectively. Its kinetic properties were also determined. The thermostability assessment showed that purified laccase is highly thermostable.
Conclusion: The results indicated that gel chromatography purification method can be an ideal to achieve high yield and fold purification of recombinant laccase with keeping enzyme properties and high thermostability, so that the recombinant purified laccase with high yield and fold can be used in drug, textile, and food industries, and environment and its detoxification.
Key words: Purification, Laccase enzyme, Trametes fungal, Physicochemical properties
Funding: This study was funded by Islamic Azad University, Central Tehran Branch.
Conflict of interest: None declared.
How to cite this article: Hesampour A, Mohandesi N. Purification of Fungal Laccase from Trametes and Characterization of Recombinant Laccase Physicochemical Properties. J Rafsanjan Univ Med Sci 2018; 17 (6): 511-22. [Farsi]
Z. Moradpour, M. Rezaei, Z. Torabi, F. Khosravi, M. Ebrahimi, Gh. Hesam,
Volume 17, Issue 11 (1-2019)
Abstract
Background and Objectives: Musculoskeletal disorders is very common in drivers of public vehicles. Therefore, the purpose of this study was to measure the correlation between muscle fatigue assessment (MFA) and Cornell musculoskeletal discomfort questionnaire (CMDQ) in taxi drivers in Shahroud in 2017.
Materials and Methods: This descriptive study was performed on 140 taxi drivers in Shahroud in 2017. Data were collected using demographic questionnaire, CMDQ and MFA chart. Statistical analysis was performed by descriptive statistics (frequency, percentage, mean) and inferential statistics (Eta coefficient).
Results: The MFA results showed that 82% of lumbar muscle fatigue level was very high. The CMDQ results also showed that most musculoskeletal disorders was in the lower back and the least musculoskeletal disorders was in the forearm. Correlation between MFA and CMDQ showed that the highest correlation was in the back region with Eta=0.574 and the lowest correlation was in the neck region with Eta=0.294.
Conclusion: The high correlation between MFA and CMDQ showed that MFA can be used instead of CMDQ. CMDQ is a self-report method and there is the possibility of non-cooperation or false report..
Key words: Muscle fatigue, Musculoskeletal disorders, Automobile driving
Funding: This research was funded by Research Committee of Shahroud University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Shahroud University of Medical Sciences approved the study (IR.SHMU.REC.1396.114).
How to cite this article: Moradpour Z, Rezaei M , Torabi Z, Khosravi F, Ebrahimi M, Hesam Gh. Study of Correlation Between Muscle Fatigue Assessment and Cornell Musculoskeletal Disorders Questionnaire in Shahroud Taxi Drivers in 2017: A Descriptive Study. J Rafsanjan Univ Med Sci 2019; 17 (11): 1031-42. [Farsi]
Abdolreza Babaee, Ebrahim Rezazadeh Zarandi, Ahmad Shabanizadeh, Zahra Taghipour, Samereh Dehghani-Soltani, Mahnaz Ramezani, Amirhesam Khodaei, Mohammad Mohsen Taghavi,
Volume 23, Issue 6 (10-2024)
Abstract
Background and Objectives: The aim of this study was to investigate the effect of sheep lymph nodes and antibiotics on the bacterium that causes acne and the bacteria that usually exist with it.
Materials and Methods: In this laboratory study, 24 patients with acne (18 to 45 years old) were selected to isolate the bacteria. Also, 24 clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis were isolated from the wound samples of patients. After culturing the bacteria, their sensitivity to common antibiotics was evaluated. Data were reported as percentage.
Results: The results showed that Cutibacterium acnes and Staphylococcus aureus were 100% sensitive to vancomycin. Also, Cutibacterium acnes showed sensitivity in tetracycline and imipenem groups in 95% of cases and in cotrimoxazole and piperacillin/tazobactam groups in 90% of cases. In addition, the highest sensitivity of Staphylococcus epidermis to piperacillin/tazobactam (62.5%) and the highest drug resistance (91.67%) were related to azithromycin and erythromycin groups.
Conclusion: Due to the increase in drug resistance, it is suggested to use antibiotics with the least drug resistance such as vancomycin and imipenem in the treatment of acne.
Keywords: Lymph node, Antibiotic, Acne, Staphylococcus aureus, Cutibacterium acnes
Funding: This study was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical considerations: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1399.103).
Authors’ contributions:
- Conceptualization: Mohammad Mohsen Taghavi, Ebrahim Rezazadeh Zarandi
- Methodology: Mohammad Mohsen Taghavi, Ahmad Shabanizadeh
- Data collection: Mahnaz Ramezani, Amir Hesam Khodaei
- Formal analysis: Abdolreza Babaee, Samereh Dehghani-Soltani
- Supervision: Mohammad Mohsen Taghavi, Zahra Taghipour
- Project administration: Mohammad Mohsen Taghavi
- Writing - original draft: Abdolreza Babaee, Ebrahim Rezazadeh Zarandi
- Writing - review & editing: Mohammad Mohsen Taghavi, Abdolreza Babaee
Citation: Babaee A, Rezazadeh Zarandi E, Shabanizadeh E, Taghipour Z, Dehghani-Soltani S, Ramezani M, Khodaei AH, Taghavi MM. Investigation of Inhibitory Effect of Sheep Lymph Nodes and Antibiotics on Cutibacterium Acnes, Staphylococcus Aureus, and Staphylococcus Epidermidis in the Laboratory Condition: A Short Report. J Rafsanjan Univ Med Sci 2024; 23 (6): 568-75. [Farsi] |
