Showing 3 results for Khanjani Jelodar
S. Khanjani Jelodar, M. Bigdeli, A. Hajizadeh Moghaddam,
Volume 19, Issue 6 (9-2020)
Abstract
Background and Objectives: The stress caused by maternal separation in the early days after birth induces autistic behaviors. Hypericin has many biological properties, including antidepressant and anti-inflammation. Therefore, this study aimed at determining hypericin effect on autism behaviors that induced by maternal separation model.
Materials and Methods: In this experimental study, Wistar rats were divided into two control and maternal separation groups. The rats in the maternal separation group were subjected to individual separation from their mother for 1 h per day (postnatal day1-9). The maternal separation group was divided into 4 subgroups: Autism, fluoxetine, hypericin (10mg/kg), hypericin (50mg/kg). Treatment was gavaged in pups on postnatal day 21-34. In the end of day 34, neurobehavioral tests (grooming, social interaction and open field test) were carried out. Statistical analysis and between group comparison were performed using one-way ANOVA followed by Tukey's test.
Results: In the pups subjected to individual isolations from their mother, social deficits (p=0.007), excessive repetitive behavior (p=0.001), and anxiety (p=0.001) were increased. Whereas, hypericin could reduce repetitive behaviors index (p=0.011), anxiety (p=0.001) and social interaction deficits (p=0.031) in the maternal isolation autism rat model.
Conclusion: These findings indicated that maternal separation may produce autistic-like behaviors, and hypericin through antidepressant effect may be a potential therapeutic agent against autistic-like behavior.
Key word: Hypericin, Maternal separation, Autistic-like behaviors, Rat
Funding: This study did not have any funds.Conflict of interest: None declared.
Ethical approval: The animal experiments were approved by the Institutional Animal Ethics Committee (IR.UMZ.REC.1397.0100), which follows the principles of the University of Mazandaran for the Use of Animals.
How to cite this article: Khanjani Jelodar S, Bigdeli M, Hajizadeh Moghaddam A. The Effect of Hypericin on Autism Like Behavior in Maternal Separation Animal Model: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (5): 569-78. [Farsi]
A. Hajizadeh Moghaddam, M. Mosavi Mehr, V. Hasantabar, S. Khanjani Jelodar,
Volume 19, Issue 7 (10-2020)
Abstract
Background and Objectives: Alzheimer’s disease (AD) is a neurodegenerative disease accompanied by common emotional symptoms such as anxiety and fear. The aim of this study was investigating the anti-anxiety and antioxidant effects of sumac extract and its nano-phytosome, in streptozotocin (STZ)-induced rat model of Alzheimer's disease
Materials and Methods: In this experimental study, 35 male Wistar rats were divided into five groups (control, positive control, Alzheimer, and Alzheimer with (40mg/kg) doses of sumac extract and nano-phytosome - sumac). For the induction of Alzheimer model, STZ (3 mg / kg) was injected intracerebroventricularlly. 21 days after gavage, plus maze test was performed to evaluate anxiety behavior in the rats. In the end, catalase and superoxide dismutase enzyme's activity and malondialdehyde levels were measured in the cerebral cortex. Statistical analysis was performed using one-way ANOVA followed by Tukey's test.
Results: The results showed that STZ increased anxiety-like behaviors (p=0.001), malondialdehyde (MDA) level (p<0.017), and decreased catalase and superoxide dismutase activity in the Alzheimer group (p<0.001); while treatment with sumac nano-phytosome reduced anxiety-like behaviors (p=0.002), increased catalase and superoxide dismutase activity and decreased MDA level in cerebral cortex (p=0.003).
Conclusion: The results suggested that sumac nano-phytosome due to improving the bioavailability may reduce STZ-induced anxiety-like behaviors and oxidative stress in rat model of AD.
Key words: Sumac nan-ophytosome, Anxiety-like behavior, Oxidative stress, Alzheimer, Rat
Funding: None declared.
Conflict of interest: There are no conflicts of interest.Ethical approval: The animal experiments were approved by the Institutional Animal Ethics Committee (IR.UMZ.REC.1397.027), which follows the principles of the University of Mazandaran for the Use of Animals.
How to cite this article: Hajizadeh Moghaddam A, Mosavi mehr M, Hasantabar V, Khanjani Jelodar S. Antioxidant and Anti-anxiety Effects of Sumac Nano-Phytosome in Rat Model of Alzheimer’s Disease: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (7): 647-60. [Farsi]
Maryam Gholizadeh, Akbar Hajizadeh Moghaddam, Farhad Valizadehgan, Sedigheh Khanjani Jelodar,
Volume 22, Issue 6 (10-2023)
Abstract
Background and Objectives: Ethanol has shown strong neurodegenerative consequences in brain that are associated with oxidative stress. The aim of this study was to investigate the effects of silymarin on oxidative stress, anxiety, and learning disorder induced by ethanol in rats.
Materials and Methods: In this experimental study, 35 male Wistar rats with 200gr average weight were divided into five groups. The control group and four groups that received 4 gr/kg ethanol for 14 days. Three treatment groups were treated with doses of 50, 100, and 150 mg/kg silymarin for 14 days, respectively. Cognitive impairment and anxiety were assessed. The average activity of catalase and superoxide dismutase enzymes and glutathione (GSH) level in the hippocampus were also assessed. Data were analyzed using one-way analysis of variance (ANOVA) and Turkey’s post hoc test.
Results: Ethanol consumption significantly decreased the discrimination index (p<0.001), the percentage of open arm time (%OAT) (p<0.001), and the percentage of open arm entry (%OAE) (p=0.007) compared to the control group. Also, it reduced the activity of antioxidants catalase (p<0.001), superoxide dismutase (p=0.002), and glutathione level (p<0.001) compared to the control group, while silymarin consumption with dose 150 mg/kg significantly increased the discrimination index, OAE (p=0.022), and OAT (p=0.023) compared to the ethanol group. Also, it increased antioxidant activity of catalase (p=0.008), superoxide dismutase (p=0.030), and glutathione (p<0.001) compared to the ethanol group.
Conclusion: Silymarin may protect the hippocampus against behavioral disorder induced by ethanol consumption by increasing the level of antioxidants.
Key words: Silymarin, Cognitive impairment, Oxidative stress, Anxiety, Hippocampus, Rat
Funding: This study was funded by Mazandaran University.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Mazandaran University approved the study (IR.UMZ.REC.1400.012).
How to cite this article: Gholizadeh Maryam, Hajizadeh Moghaddam Akbar, Valizadegan Farhad, Khanjani Jelodar Sedigheh Silymarin Antioxidant Effect on Ethanol-Induced Anxiety and Learning Impairments in Rats: An Experimental Study. J Rafsanjan Univ Med Sci 2023; 22 (6): 555-66. [Farsi]
