Journal of Rafsanjan University of Medical Sciences

---

Background and Objective: Aloe-emodin is a Hydroxyanthraquinone that exists in Aloe Vera leaves and has been known to have anti-tumor effects. Various studies have mentioned the biological effects of Aloe Vera such as antiviral, antimicrobial and some hepato-protective effects. Recently its antitumor effects have attracted the attention of many researchers. Breast cancer is the most common malignancy in women, accounting for 30% of all female cancers and < 1% of all cancer cases in men. Breast cancer is also responsible for 15% of cancer deaths in women. The aim of the present study was to investigate the anti-cancer effects of aloe-emodin on human breast cancer MCF-7 cell line.

Materials and Methods: In this laboratory study MCF-7 cells were cultured in RPMI medium with 10% fetal bovine serum (FBS). The cytotoxic effect of different concentrations (5, 10, 25, 50, and 100 µM) of Aloe-emodinon cultured cells were evaluated by MTT assay at different times (24, 48, 72 h). Apoptosis and CD95 (Fas) expression were analyzed by flow-cytometry using an Annexin V-FITC/PI kit and Fas (CD95) kit according to the manufacturer’s protocol.

Results: Aloe-emodin decreases the viability of MCF-7 cell line in a time and dose dependent manner, so that the most effective concentration of this substance was 100µM and 72 h after treatment. According to the data of Fas (CD95) expression and Annexin-PI, the highest apoptosis induction rate was seen in 100µM and 72 h.

Conclusion: The findings of the present study indicated that Aloe-emodinhas has some antitumor effects and can be used in the treatment of breast cancer. However, further investigation of its cytotoxic effects against tumor cells, both in vitro and in vivo, is recommended.

Key words: Breast Cancer, Aloe-emodin, Apoptosis, MCF-7

 

Funding: This research was funded by Rafsanjan University of Medical Sciences.

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.

 

How to cite this article:  Hosseini J, Mahmoodi M, Hakhamaneshi M, Jalili A, Khoshdel A, Sheikhfathollahi M, Fakhari Sh, Hosseini-zijoud SM. Apoptosis Effects of Aloe-emodin against MCF-7 Cell Line J Rafsanjan Univ Med Sci 2014 13(1): 41-52. [Farsi]

---

Background and Objective: Recently, it is considered the role of hypoglycemic agents specially medicinal plants derivatives in treatment of diabetes mellitus. Aloe Vera contains phenolic compounds and flavonoids with antioxidant properties that reduces the complications of the disease.The aim of study was to investigate the effects of hydroalcoholic extract of Aloe Vera in serum levels of glucose and insulin and key enzymes in metabolic pathways of glycolysis and gluconeogenesis in streptozotocin-induced diabetic Wistar rats.

Materials and Methods: A total of 50 rats (initial weight of 220 ±10 g) were divided into five groups. Two months after treatment with Aloe Vera extract, the rats were sacrificed and blood glucose and serum insulin levels were measured. It was also isolated the liver then, extracted mRNA from it, expression levels of Glucokinase and Phosphoenol pyruvate carboxy kinase enzymes were measured by Real Time PCR.

Results: The results showed that the extract of Aloe Vera at doses of 100, 200 and 400 mg/kg increase insulin secretion (p<0.05), hypoglycemia (p<0.001), increasing expression of Glucokinase enzyme (p<0.05) and reducing  expression of the Phosphoenol pyruvate carboxy kinase enzyme (p<0.05) in diabetic rats.

Conclusion: Not only dose Aloe Vera hydroalcoholic extract influence on insulin secretion, it also affects on key enzymes gene expression of Glycolysis and Gluconeogenesis pathways. Hence, this extract can be effective for improvement of diabetic complications.

Key words: Glycolysis and Gluconeogenesis pathways key enzymes, Streptozotocin, Aloe Vera, Rat

 

Funding: This research was funded by Rafsanjan University of Medical Sciences.

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.

 

How to cite this article: Chahardoli M, Mahmoodi M, Hajizadeh MR, Khoramdel Azad H, Khoshdel AR, Mirzae MR. Effect of Aloe Vera Hydroalcoholic Extract on Blood Glucose, Serum Insulin and the Key Enzymes in Metabolic Pathways of Glycolysis and Gluconeogenesis in Hepatocytes of Type 1 Diabetic Rats. J RafsanjanUniv Med Sci 2014 13(8): 668-82 [Farsi]

---

Background and Objectives: Diabetes mellitus is characterized as one of the most common endocrine diseases throughout the world. The use of antioxidants is the most preferred way to decrease complications of diabetes mellitus. Several studies showed the antioxidant effects of walnut leaf. Therefore, the aim of the present work was to survey the effects of walnut leaf animal diet on some blood biochemical metabolites in diabetic rats.

 Materials and Methods: In this experimental study, 40 albino male rats (250-300 gram body weight) were divided into five groups of 8. Diabetes was induced by an intraperitoneal injection of streptozotocin (STZ) (50 mg/kg). Three groups of Diabetic rats received 1%, 2% and 5% weight concentration of walnut leaf powder in daily diets. The study was performed during 28 days. The blood samples were collected at the beginning and the end of study, and biochemical parameters including HbA1c, FBS, TG, HDL-C, LDL-C, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assayed. Statistically, the quantitative data obtained, compared among the groups by ANOVA. Statistical significance was considered at p<0.05.

 Results: The results of the current study showed that consumption of walnut leaf decreased TG, ALT, ALP, and HbA1c levels significantly. The consumption of 5% walnut leaf had the most effects. But it had no significant effect on FBS.

Conclusion: According to the results of the present study, it can be concluded that walnut leaf has useful effects on HbA1c and ALP levels and 5% of walnut leaf powder showed the most effect.

Key words: Diabetes Mellitus, Walnut Leaf, Antioxidant, Hyperglycemia, HbA1c, Rat

Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.

Funding: This research was funded by Rafsanjan University of Medical Sciences.

Conflict of interest: None declared.

How to cite this article: Mahmoodi M, Fahmidehkar MA,. Rezaeian, M.R. Hajizadeh, A. Khoshdel, A. Pourrashidi Boshrabadi. Study of the Effects of Walnut Leaf on the Levels of a Number of Blood Biochemical Factors in Diabetic Male Rats. J Rafsanjan Univ Med Sci 2016; 15(8): 753-64. [Farsi]

---

Background and Objectives: Type 1 diabetes (diabetes mellitus) is a disease associated with metabolism. The most common form of type 1 diabetes is its autoimmune type. Alpha-1-antitrypsin (AAT) is a member of the serine protease inhibitors family and its role is to protect tissue degradation by protease. Hence, its defect or deficiency significantly increases the risk of various diseases. This study aimed to compare the activity of alpha-1 antitrypsin protein in patients with type 1 diabetes with healthy individuals.
Materials and Methods: This descriptive study carried out on 42 patients with type 1 diabetes between the ages of 18-28 years old who  referred to the Diabetes Clinic in the city of Rafsanjan in 2015. Healthy people were chosen among native male and female students aged from 18 to 28 years of Rafsanjan University of Medical Sciences who were matched for age and sex with the patients. AAT activity by trypsin inhibitory capacity (TIC) was  studied. For statistical analysis the chi-square test, t-test, and Pearson correlation coefficient were used.
Results: The amount of TIC in the patients with type 1 diabetes was 2.35 ±0.40 (μmol/min/ml) which was significantly lower than the amount in the healthy individuals that was 3.36 ±0.36 (μmol/min/ml) (P-value<0.001).
Conclusion: The results of this study revealed that AAT activity in the patients with type 1 diabetes was lower than  the healthy individuals. Therefore, it can be stated that AAT can be introduced as a potential target for treatment of type 1 diabetes.
Key word: Type 1 diabetes, Alpha-1 Antitrypsin, Trypsin Inhibitory Capacity
 
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study.
 
How to cite this article: Ghoreishi A.S., Mahmoodi  M, Hajizade M.R, Sheykhfatollahi M, Shafipour M.R., Khoshdel A.R. The Comparison of Alpha 1- Antitrypsin Protein Activity in Type 1 Diabetic Patients Referred to the Rafsanjan Diabetes Center to Healthy Individuals in 2016. J Rafsanjan Univ Med Sci 2018; 16(11): 53-62. [Farsi]
 

---

Background and Objectives: Musculoskeletal disorders are among the most common injuries in dentists. Therefore, the present study was conducted to determine the relationship between students' level of knowledge of the principles of ergonomics and body position during dental treatments.
Materials and Methods: In this descriptive study, 41 dental students of Guilan University of Medical Sciences at the clinical level were enrolled in 2017. Ergonomic condition was assessed using Rapid Entire Body Assessment (REBA) standard index. Data were analyzed using Spearman’s correlation coefficient test and independent t-test.
Results: The results showed 21 (51.2%) students were at high and also 15 (36.6 %) students were at very high-risk levels. Also a negative correlation was found between the level of knowledge and REBA score (r=-0.137, p=0.036).
Conclusion: Most students were at high risk for musculoskeletal problems and low ergonomic awareness. The results of the study raise the need for ergonomic knowledge training.
Key words: Knowledge, Dental Students, Ergonomics, Posture, Guilan
 
Funding: This study did not have any funds.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Guilan University of Medical Sciences approved the study (IR.GUMS.REC.1395/391).
 
How to cite this article: Mirfarhadi N, Radafshar G, Ghodousian S, Khoshdel Salakjani M. The Relationship between Knowledge of Ergonomics and Body Position during Clinical Procedures among Dental Students of Guilan University of Medical Sciences in 2017: A Short Report. J Rafsanjan Univ Med Sci 2021; 19 (12): 1319-28. [Farsi]