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Characterization and Evaluation of Antimicrobial Effects of ZnO/Ag Nanoparticles Synthesized by Milk Thistle Seed Extract (Silybum marianum): A Short Report
A. Bayrami, F. Mohammadi Arvanagh, S. Zahri, M. Bayrami,
Volume 19, Issue 5 (8-2020)
Abstract
 
Background and Objectives: The use of nanotechnology is rapidly expanding in various fields, especially in the health and pharmaceutical fields. The purpose of this study was to produce biological Zinc Oxide (ZnO) and silver (Ag) nanoparticles (NPs) using extract of milk thistle seeds and to determine their antibacterial properties.
Materials and Methods: In this laboratory study, biological NPs were synthesized using aqueous extract of milk thistle, and ZnO NPs were chemically synthesized as well. After investigating the structural properties, the antibacterial effects of the nanomaterials on Escherichia coli and Staphylococcus aureus were studied at the concentrations of 0.8, 0.4, 0.2, 0.1, 0.05 and 0 (control) mg/ml.
Results: The size of the biologically synthesized ZnO/Ag NPs was determined (17.5 nm), which was smaller than the chemical NPs (22 nm). Due to smaller size, it could have more bioactivity. Furthermore, the presence of organic compounds in the structure of NPs was confirmed. Both types of NPs showed dose-dependent antibacterial effects. Despite high sensitivity of bacteria to the biological NPs, this difference was statistically significant only at theconcentration of 0.8 mg/ml (p<0.05).
Conclusion: The findings showed that biological NPs were more effective on the bacterial growth. This could be due to their smaller size and presence of active plant compounds in their structure.
Key words: Milk thistle, Antibacterial, Green synthesis, ZnO/Ag NPs
 
Funding: This study was funded by University of Mohaghegh Ardabili, Ardabil, Iran.
Conflict of interest: None declared.
Ethical  approval[j1] : The Ethics Committee of University of Mohaghegh Ardabili approved the study.
 
How to cite this article: Bayrami A, Mohammadi Arvanagh F, Zahri S, Bayrami Z. Characterization and Evaluation of Antimicrobial Effects of ZnO/Ag Nanoparticles Synthesized by Milk Thistle Seed Extract (Silybum marianum): A Short Report. J Rafsanjan Univ Med Sci 2020; 19 (5): 539-48. [Farsi]
 [j1]کد اخلاق گزارش نشده است.

Evaluating the Efficiency of Selenium Nanoparticles in the Production of Decellularized Neural Scaffold and the Ability to Preserve Stem Cells Cultured on Them: A Laboratory Study
A. Gholami, A. Asadi, A. Abdolmaleki, S. Zahri,
Volume 20, Issue 7 (10-2021)
Abstract
Background and Objectives: Selenium is a trace element and its nanoparticle has antimicrobial activity and due to their low toxicity and excellent biological performance, it has many biological applications in tissue engineering. The aim of this study was to investigate the effect of selenium nanoparticles on stem cell interaction on rat decellularized sciatic nerve scaffolds.
Materials and Methods: In this laboratory study, the effect of selenium nanoparticles on stem cell interaction on rat decellularized sciatic nerve scaffold, scaffolds were prepared using Sondell decellularizaton method and stored in PBS solution containing antibiotics. Biomechanical and histological evaluations of scaffolds were examined using the light microscope. In the next step, adipose stem cells were seeded on the scaffold and the viability of the cells on the scaffold in the presence of selenium nanoparticles were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. Data were analyzed by one-way analysis of variance and Tukey's post hoc test.
Results: The tensile test showed that after decellularization, extracellular matrix components such as collagen, laminin, and elastin were retained in the scaffold. Also, DNA content was significantly reduced in the scaffold group (p<0.01). The results of MTT test showed that selenium nanoparticles had no toxicity on stem cells cultured on the scaffold.
Conclusion: The biocompatibility of scaffolds and the viability of stem cells treated with selenium nanoparticles were not significantly different from the control group. Therefore, it can be evaluated as a reinforcing factor to increase the efficiency of scaffolds for the use in regeneration of the nerve lesions.
Key words: Selenium nanoparticles, Stem cell, Decellularized scaffold, Regeneration, Tissue engineering
 
Funding: This study was funded by University of Mohaghegh Ardabili.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of University of Mohaghegh Ardabili approved the study (IR.UMA.REC.1397.195).
 
 
How to cite this article: Gholami A, Asadi A, Abdolmaleki A, Zahri S. Evaluating the Efficiency of Selenium Nanoparticles in the Production of Decellularized Neural Scaffold and the Ability to Preserve Stem Cells Cultured on Them: A Laboratory Study. J Rafsanjan Univ Med Sci 2021; 20 (7): 733-46. [Farsi]
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