Volume 12, Issue 8 (10-2013)
JRUMS 2013, 12(8): 611-620 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rajaei F, Mahmoudi R, Pakdelnia A. Dexamethasone Effects on Morphometric Epididymis and
Deferens Duct on Mouse. JRUMS 2013; 12 (8) :611-620
URL: http://journal.rums.ac.ir/article-1-1393-en.html
URL: http://journal.rums.ac.ir/article-1-1393-en.html
Qazvin University of Medical Sciences
Abstract: (4228 Views)
Background and Objective: Dexamethasone is a widely use glucocorticoid and its prescription has been increased during the recent years. In the present study the effects of dexamethasone on the reproductive system of male mice was investigated.
Materials and Methods: In this experimental study, 50 adult male NMRI mice were randomly divided into five groups as experimental, sham and control. Experimental groups were given dexamethasone at a dose of 4 mg/kg, 7 mg/kg and 10 mg/kg respectively by intraperitoneally injection on 7 consecutive days. Sham group was injected with saline while control group received nothing. One day after the last injection, all animals were anesthetized. The mice and their testes were weighed. After preparation of microscopic slides, height of the epididymis and deferens epithelium was determined using Image tool software. The data were analyzed statistically using ANOVA and Scheffe test.
Results: The mean height of epididymis and deferens duct epithelium in experimental groups significantly decreased compared to sham and control group (p=0.001). Meanwhile, the severity of this reduction was dose-dependent and in 7 mg/kg group was significantly reduced. Although, the mean testicular weight of mice in experimental groups showed no significant differences compared to control group the mean weight of animals only in 7 mg/kg group significantly decreased compared to sham and control group (p=0.042).
Conclusion: The present study indicated that repeated injections of dexamethasone resulted in detrimental effects on the reproductive system by numerous changes in the epididymis and deferens duct and fertility of male mice consequently.
Key words: Dexamethasone, Epididymis, Deference duct, Mice
Funding: This research was funded by Qazvin University of Medical Sciences.
Ethical approval: None declared.
Conflict of interest: The Ethics Committee of Qazvin University of Medical Sciences approved the study.
How to cite this article: Rajaei F, Mahmoudi R, Pakdelnia A. Dexamethasone Effects on Morphometric Epididymis and Deferens Duct on Mouse. J Rafsanjan Univ Med Sci 2013; 12(8): 611-20. [Farsi]
Materials and Methods: In this experimental study, 50 adult male NMRI mice were randomly divided into five groups as experimental, sham and control. Experimental groups were given dexamethasone at a dose of 4 mg/kg, 7 mg/kg and 10 mg/kg respectively by intraperitoneally injection on 7 consecutive days. Sham group was injected with saline while control group received nothing. One day after the last injection, all animals were anesthetized. The mice and their testes were weighed. After preparation of microscopic slides, height of the epididymis and deferens epithelium was determined using Image tool software. The data were analyzed statistically using ANOVA and Scheffe test.
Results: The mean height of epididymis and deferens duct epithelium in experimental groups significantly decreased compared to sham and control group (p=0.001). Meanwhile, the severity of this reduction was dose-dependent and in 7 mg/kg group was significantly reduced. Although, the mean testicular weight of mice in experimental groups showed no significant differences compared to control group the mean weight of animals only in 7 mg/kg group significantly decreased compared to sham and control group (p=0.042).
Conclusion: The present study indicated that repeated injections of dexamethasone resulted in detrimental effects on the reproductive system by numerous changes in the epididymis and deferens duct and fertility of male mice consequently.
Key words: Dexamethasone, Epididymis, Deference duct, Mice
Funding: This research was funded by Qazvin University of Medical Sciences.
Ethical approval: None declared.
Conflict of interest: The Ethics Committee of Qazvin University of Medical Sciences approved the study.
How to cite this article: Rajaei F, Mahmoudi R, Pakdelnia A. Dexamethasone Effects on Morphometric Epididymis and Deferens Duct on Mouse. J Rafsanjan Univ Med Sci 2013; 12(8): 611-20. [Farsi]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
