Background and
Objective: Nitric oxide mediated some behavioral effects of nicotine and
ethanol in the nucleus accumbens. In the present study, the effects of L-NAME (nitric
oxide synthase inhibitor) in the nucleus accumbens on nicotine’s effect in
ethanol-induced amnesia were investigated.
Materials and Methods: In this experimental study 96
adult male wistar rats were used (12 groups). It was used inhibitory avoidable
memory step-through model in order to assess memory. The step-through latency
for the assessment of memory measured in male Wistar rats. Cannula were
bilaterally implanted in the NAC shell of all animals. One-way analysis of
variance (ANOVA) followed by multiple comparisons of Tukey test,
were used for data analysis. p<0.05 was statistically considered significant
difference among groups.
Results: Ethanol induced amnesia (p<0.001)
and state-dependent learning (p<0.001). Nicotine injection (p<0.001) or
L-NAME (p<0.010) before the test prevented amnesia. Co-administration of an
ineffective dose of nicotine with L-NAME potentiated improving effect of L-NAME
on memory impaired by ethanol (p<0.001). On the other hand,
pre-test intra-NAc injection of L-NAME impairs inhibitory avoidable
memory by itself (p<0.001).
Conclusion: The results indicated that L-NAME in the nucleus accumbens potentiated
improving effect of nicotine on the ethanol-induced amnesia.
Key words: Nitric oxide synthase, Nicotine, Ethanol amnesia,
Nucleus accumbens, Rat
Funding: This
research was funded by Lahijan Branch of Islamic Azad University.
Conflict interest: None declared.
Ethical approval: The Ethics Committee of Tehran University of Medical
Sciences approved the study.
How to cite this article:
Piri M, Moshfegh A, Raoufi N, Shahin MS. Influence of Nitric
Oxide Synthase Inhibitor in the Nucleus Accumbens on Improving Effect of
Nicotine in Ethanol-Induced Amnesia. J
RafsanjanUniv Med Sci 2014 13(5): 435-44. [Farsi]
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