Volume 19, Issue 9 (12-2020)
JRUMS 2020, 19(9): 969-978 |
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Hassanshahi J, rahmani M R, Shibani F, Kaeidi A. The Oleuropein Effect on Neurotrophic Factor Expression Level in Hippocampal Tissue and Memory Defect Induced by Morphine in Male Rats: An Experimental Study. JRUMS 2020; 19 (9) :969-978
URL: http://journal.rums.ac.ir/article-1-5268-en.html
URL: http://journal.rums.ac.ir/article-1-5268-en.html
Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abstract: (1961 Views)
Background and Objectives: Taking morphine can cause damage to the hippocampus. Oleuropein has a protective effect on brain neurons. The aim of this study was to determine the effect of oleuropein on brain-derived neurotrophic factor (BDNF) protein expression level in hippocampal tissue and memory defect induced by morphine in rats.
Materials and Methods: In this experimental study, 40 male Wistar rats were used. The animals were divided into five groups (n=8) including control/saline group (receiving saline intraperitoneally for four weeks), morphine group (receiving 40 mg/kg morphine daily subcutaneously for four weeks) and morphine + Oleuropein groups (receiving doses of 5, 15, and 30 mg/kg oleuropein + morphine by intraperitoneal injection for 4 weeks). At the end, the animals’ working memory was evaluated using the Y-maze behavior measurement test. The hippocampal BDNF protein expression level was measured using the ELISA method. One-way ANOVA test with Tukey’s post-test was used to analyze the data.
Results: Intraperitoneal injection of oleuropein at a dose of 30 mg/kg in the rats receiving morphine significantly improved the memory function (p<0.01) and increased the BDNF protein expression level (p<0.05) compared to the morphine treated groups.
Conclusion: Oleuropein may increase the BDNF protein expression level in the hippocampal tissue and improves the memory defect induced by morphine in rats.
Key words: Morphine, Oleuropein, Working memory, Y-maze test, BDNF protein
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1395.103).
How to cite this article: Hassanshahi J, Rahmani M R, Shibani F, Kaeidi A. The Oleuropein Effect on Neurotrophic Factor Expression Level in Hippocampal Tissue and Memory Defect Induced by Morphine in Male Rats: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (9): 969-78. [Farsi]
Materials and Methods: In this experimental study, 40 male Wistar rats were used. The animals were divided into five groups (n=8) including control/saline group (receiving saline intraperitoneally for four weeks), morphine group (receiving 40 mg/kg morphine daily subcutaneously for four weeks) and morphine + Oleuropein groups (receiving doses of 5, 15, and 30 mg/kg oleuropein + morphine by intraperitoneal injection for 4 weeks). At the end, the animals’ working memory was evaluated using the Y-maze behavior measurement test. The hippocampal BDNF protein expression level was measured using the ELISA method. One-way ANOVA test with Tukey’s post-test was used to analyze the data.
Results: Intraperitoneal injection of oleuropein at a dose of 30 mg/kg in the rats receiving morphine significantly improved the memory function (p<0.01) and increased the BDNF protein expression level (p<0.05) compared to the morphine treated groups.
Conclusion: Oleuropein may increase the BDNF protein expression level in the hippocampal tissue and improves the memory defect induced by morphine in rats.
Key words: Morphine, Oleuropein, Working memory, Y-maze test, BDNF protein
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Rafsanjan University of Medical Sciences approved the study (IR.RUMS.REC.1395.103).
How to cite this article: Hassanshahi J, Rahmani M R, Shibani F, Kaeidi A. The Oleuropein Effect on Neurotrophic Factor Expression Level in Hippocampal Tissue and Memory Defect Induced by Morphine in Male Rats: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (9): 969-78. [Farsi]
Type of Study: Research |
Subject:
Physiology
Received: 2020/04/27 | Accepted: 2020/12/20 | Published: 2020/12/20
Received: 2020/04/27 | Accepted: 2020/12/20 | Published: 2020/12/20
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