Volume 12, Issue 4 (6-2013)
JRUMS 2013, 12(4): 259-270 |
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The Effect of Maternal Nicotine Exposure on Fibronectin Expression in Lung Parenchyma of the Mice Newborns. JRUMS 2013; 12 (4) :259-270
URL: http://journal.rums.ac.ir/article-1-5299-en.html
URL: http://journal.rums.ac.ir/article-1-5299-en.html
Abstract: (1517 Views)
Background and Objectives: Nicotine is one of the chemical substances with high level of toxicity. It crosses the placenta and accumulates in the developing organs of fetus. In this study, the effects of maternal nicotine exposure on fibronectin expression in the lungs of newborn mice were evaluated.
Materials and Methods: Pregnant Balb/C mice were divided into 2 experimental and 2 control groups. The experimental group1, received 3 mg/kg intra peritoneal nicotine from day 7 of gestation to the last day of pregnancy. The experimental group 2 received the same amount of nicotine during the same gestational days as well as 2 first weeks after birth (lactation). The control groups received the same volume of normal saline during the same periods(n=6). At the end of exposure times, all of newborns were anesthetized and their lungs removed for histochemical and Immunohistochemical methods. Data were analyzed by student t-test and Mann-Whitney test.
Results: Our results indicated that ratios of lung weight and body weight in the offsprings born to the mothers with nicotine exposure compared to that of the control groups were significant. Histochemical study showed that pneumocytes type 1 differentiation and alveoli diameter were decreased in the experimental groups. Fibronectin immunoreactivity intensity was not similar in the different parts of the lungs including alveoli, bronchiole and small vessels. A significant increase in immunoreactivity showed that in alveoli experimental group 2, but in bronchioles and vessels of the experimental groups were decreased in contrast to the control groups (p<0.05).
Conclusion: These data also indicated that maternal nicotine exposure may induce abnormal fibronectin expression which might cause defects in lung function during life time..
Key words: Nicotine, Fibronectin, Lungs, Offspring mouse
Funding: This research was funded by Mashhad University of Medical Sciences.
Conflict of interest: None declared.
Ethical Approval: The Ethics Committee of Mashhad University of Medical Scinces approved the study.
How to cite this article: M. Shariati Kohbanani, M.R. Nikravesh, M. Jalali, M. Sankian, A.R Fazel, A.R. Ebrahimzadeh. The effect of maternal nicotine exposure on fibronectin expression in lung parenchyma of the mouse newborns. J Rafsanjan Univ Med Scie 2013; 12(4): 259-70. [Farsi]
Materials and Methods: Pregnant Balb/C mice were divided into 2 experimental and 2 control groups. The experimental group1, received 3 mg/kg intra peritoneal nicotine from day 7 of gestation to the last day of pregnancy. The experimental group 2 received the same amount of nicotine during the same gestational days as well as 2 first weeks after birth (lactation). The control groups received the same volume of normal saline during the same periods(n=6). At the end of exposure times, all of newborns were anesthetized and their lungs removed for histochemical and Immunohistochemical methods. Data were analyzed by student t-test and Mann-Whitney test.
Results: Our results indicated that ratios of lung weight and body weight in the offsprings born to the mothers with nicotine exposure compared to that of the control groups were significant. Histochemical study showed that pneumocytes type 1 differentiation and alveoli diameter were decreased in the experimental groups. Fibronectin immunoreactivity intensity was not similar in the different parts of the lungs including alveoli, bronchiole and small vessels. A significant increase in immunoreactivity showed that in alveoli experimental group 2, but in bronchioles and vessels of the experimental groups were decreased in contrast to the control groups (p<0.05).
Conclusion: These data also indicated that maternal nicotine exposure may induce abnormal fibronectin expression which might cause defects in lung function during life time..
Key words: Nicotine, Fibronectin, Lungs, Offspring mouse
Funding: This research was funded by Mashhad University of Medical Sciences.
Conflict of interest: None declared.
Ethical Approval: The Ethics Committee of Mashhad University of Medical Scinces approved the study.
How to cite this article: M. Shariati Kohbanani, M.R. Nikravesh, M. Jalali, M. Sankian, A.R Fazel, A.R. Ebrahimzadeh. The effect of maternal nicotine exposure on fibronectin expression in lung parenchyma of the mouse newborns. J Rafsanjan Univ Med Scie 2013; 12(4): 259-70. [Farsi]
Type of Study: Research |
Subject:
علوم تشريح
Received: 2020/05/9 | Accepted: 2020/05/9 | Published: 2020/05/9
Received: 2020/05/9 | Accepted: 2020/05/9 | Published: 2020/05/9
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