Journal of Rafsanjan University of Medical Sciences

  In Vitro Antioxidant Activity of Pyrus Boissieriana, Diospyros Lotus, Eryngium Caucasicum and Froriepia Subpinnata

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  S.M Nabavi ^[1] , S.F. Nabavi¹, M.A. Ebrahimzadeh [2] , B. Eslami [3]

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  Received: 02/11/08 Sent for Revision: 25/01/09 Received Revised Manuscript: 25/05/09 Accepted: 08/06/09

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  Background and Objectives: Pyrus boissieriana, Diospyros lotus, Eryngium caucasicum, Froriepia subpinnata are native to Northern part of Iran and are widly used in local foods. Diospyros lotus fruits are used in prepration of jams. E.caucasicum and F.subpinnata also used as wild herbs in local foods.

  Materials and Methods: In this Experimental study, the antioxidant activity of methanolic extract of Pyrus boissieriana, Diospyros lotus, Eryngium caucasicum, Froriepia subpinnata were determined with 6 invitro assay include DPPH, nitric oxide, H₂O₂ radicals scavenging, linoleic acid, reucing power and iron ion chelating activity. Total phenolic and flavonoid compounds of extract was measured by Folin Ciocalteu and AlCl₃ assays .

  Results: IC₅₀ in DPPH method was in order : E. caucasicum > F. subpinnata > D. lotus > and P. boissieriana mg ml^-1, respectively . The extracts showed weak nitric oxide-scavenging activity. The E. caucasicum and F. subpinnata extracts were better than others respectively. The F. subpinnata and E. caucasicum had shown better reducing power than other extract that was comparable with Vit C (p<0.05).

  Conclusion: F.subpinnata aeial parts and E. caucasicum leaves had higher total phenolic and flavonoid contents than others, which may be the result of higher total phenolic and flavonoid contents of them.

  Keywords: Antioxidant, Eryngium. Caucasicum, Froriepia. Subpinnata, Pyras boissieriana. lotus

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  Funding: This research was funded by a grant from the research council of Mazandaran University of Medical Sciences.

  Conflict of interest: None declared.

  Ethical approval: The Ethics Committee of Mazandaran University of Medical Sciences approved the study .

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  [1] - Researcher, Pharmaceutical Sciences^�Research Center, University of Medical Sciences, Mazandaran, Iran

  [2] - Aassociate Prof. Dept of Medicinal Chemistry, Pharmaceutical Sciences^�Research Center, University of Medical Sciences, Mazandaran, Iran

  (Corresponding Author) Tel: (0151) 3543081, Fax: (0151) 3543084, E-mail: zadeh20 @yahoo.com

  [3] - Assistante Prof., Dept. of Plant Systematic, Islamic Azad University of Ghaemshahr, Iran