Journal of Rafsanjan University of Medical Sciences

Background and Objectives: Chemotherapy has many side effects on tissues such as the liver.The aim of this study was to evaluate the effect of aerobic exercise and curcumin on gene expression of apoptotic markers of liver tissue of cancer mice in doxorubicin treatment phase.
Materials and Methods: The current research is of experimental type. 36 female Balb/c mice weighing 20±2 grams were randomly divided into six groups: G1 healthy control, G2 patient control group, G3 patient+treatment (doxorubicin drug use), G4 patient+treatment+aerobic exercise, G5 patient+treatment+supplement group, and G6 patient+treatment+supplement+aerobic exercise group. 4T1 cell line was used to induce breast cancer. Doxorubicin was injected intraperitoneally on days 1, 7, 14, 21, and 28, and curcumin was fed to the animals. Aerobic training consisted of 30 minutes of running with an intensity of 40-60% of maximum speed, five days a week for 6 weeks. In the first week, the exercises reached a speed of 14 meters per minute, and in the second week it reached 16 meters and finally 18 meters per minute. One-way analysis of variance was used for data analysis.
Results: The effect of curcumin and aerobic exercise on the expression of Cas3 (p=0.0001) and Bcl2 (p=0.0001) genes was statistically significant. In group 6, Bcl2 gene expression increased compared to other groups, and Cas3 decreased in groups 5 and 6 compared to group 2.
Conclusion: Probably aerobic exercise combined with the use of nanocurcumin in the treatment phase with doxorubicin provides the necessary adaptations to inhibit or stop apoptosis in the liver tissue of mice with breast cancer.
Key words: Aerobic exercise, Curcumin, Apoptosis index, Breast cancer, Liver, Doxorubicin

Funding: This study did not have any funds.
Conflict of Interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University of Parand of approved the study (IR.IAU.PIAU.REC.1400.019) 
How to cite this article: Sadeghian Solmaz, Kazemzadeh Yaser, Yahya Mohammadnejadpanah Kandi Yahya, Mirzayan Shanjani Sanaz, Sedaghati Saeed. The Effect of Aerobic Exercise with Curcumin Consumption on Tissue Apoptosis Indices in the Liver Tissue of Rats Induced by Breast Cancer in the Doxorubicin Treatment Phase: An Experimental Study. J Rafsanjan Univ Med Sci 2022; 21 (4): 433-48. [Farsi]