Volume 21, Issue 11 (2-2023)
JRUMS 2023, 21(11): 1133-1152 |
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Ethics code: IR.IAU.Kerman.REC.1400.001
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Hosseini-Naveh O, Kariminik A, Soltani-Nezhad S, Ranjbar M, Sheikhhosseini E. Isolation, Identification, and Investigation of Biological Properties of Biosurfactant-Producing Probiotics and Magnesium Nanoparticles from Local Dairy Samples: A Laboratory Study. JRUMS 2023; 21 (11) :1133-1152
URL: http://journal.rums.ac.ir/article-1-6735-en.html
URL: http://journal.rums.ac.ir/article-1-6735-en.html
Ozra Hosseini-Naveh 
, Ashraf Kariminik , Shahla Soltani-Nezhad , Mehdi Ranjbar , Enayatollah Sheikhhosseini
, Ashraf Kariminik , Shahla Soltani-Nezhad , Mehdi Ranjbar , Enayatollah Sheikhhosseini
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran.
Abstract: (377 Views)
Background and Objectives: Probiotics are common and well-known microorganisms in human and animal microflora and have been widely studied for medical and food productions. The present research was conducted with the aim of isolating and identifying probiotic bacteria and the ability to produce biosurfactant and magnesium oxide nanoparticles from local dairy products in Rafsanjan, Iran.
Materials and Methods: In this laboratory study, the local dairy samples were transported to the laboratory under sterile conditions, observing the cold chain, and cultured in MRS (de Man Rogosa Sharpe) agar medium. In order to isolate biosurfactant-producing isolates, oil spreading and emulsification tests were performed, then the isolates were further studied in terms of the production of magnesium oxide nanoparticles. The size and morphology of nanoparticles, antioxidant activity, FTIR (Fourier Transform Infrared Spectroscope), and their antibacterial effects were determined. Then polymerase chain reaction, sequencing, and phylogeny tree drawing were performed for the identification of superior strain.
Results: Nine probiotic isolates had the ability to produce biosurfactants, all of which had a significant emulsifying activity. The produced biosurfactants had antibacterial properties. One of the biosurfactant-producing isolates was able to produce magnesium oxide nanoparticles, which was named Enterococcus faecium L8 based on the molecular identity. The size of 90% of nanoparticles was 107 nm, which showed significant antibacterial and antioxidant effects.
Conclusion: Enterococcus faecium L8 has a favorable probiotic ability, and this native strain can be used in the formulation of multipurpose foods by conducting more tests.
Key words: Traditional dairy products, Probiotics, Biosurfactant, Magnesium oxide nanoparticles
Funding: This study did not have any funds.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University of Kerman approved the study (IR.IAU.Kerman.REC.1400.001).
How to cite this article: Hosseini-Naveh Ozra, Kariminik Ashraf, Soltani-Nezhad Shahla, Ranjbar Mehdi, Sheikhhosseini Enayatollah. Isolation, Identification, and Investigation of Biological Properties of Biosurfactant-Producing Probiotics and Magnesium Nanoparticles from Local Dairy Samples: A Laboratory Study. J Rafsanjan Univ Med Sci 2023; 21 (11): 1133-52. [Farsi]
Materials and Methods: In this laboratory study, the local dairy samples were transported to the laboratory under sterile conditions, observing the cold chain, and cultured in MRS (de Man Rogosa Sharpe) agar medium. In order to isolate biosurfactant-producing isolates, oil spreading and emulsification tests were performed, then the isolates were further studied in terms of the production of magnesium oxide nanoparticles. The size and morphology of nanoparticles, antioxidant activity, FTIR (Fourier Transform Infrared Spectroscope), and their antibacterial effects were determined. Then polymerase chain reaction, sequencing, and phylogeny tree drawing were performed for the identification of superior strain.
Results: Nine probiotic isolates had the ability to produce biosurfactants, all of which had a significant emulsifying activity. The produced biosurfactants had antibacterial properties. One of the biosurfactant-producing isolates was able to produce magnesium oxide nanoparticles, which was named Enterococcus faecium L8 based on the molecular identity. The size of 90% of nanoparticles was 107 nm, which showed significant antibacterial and antioxidant effects.
Conclusion: Enterococcus faecium L8 has a favorable probiotic ability, and this native strain can be used in the formulation of multipurpose foods by conducting more tests.
Key words: Traditional dairy products, Probiotics, Biosurfactant, Magnesium oxide nanoparticles
Funding: This study did not have any funds.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University of Kerman approved the study (IR.IAU.Kerman.REC.1400.001).
How to cite this article: Hosseini-Naveh Ozra, Kariminik Ashraf, Soltani-Nezhad Shahla, Ranjbar Mehdi, Sheikhhosseini Enayatollah. Isolation, Identification, and Investigation of Biological Properties of Biosurfactant-Producing Probiotics and Magnesium Nanoparticles from Local Dairy Samples: A Laboratory Study. J Rafsanjan Univ Med Sci 2023; 21 (11): 1133-52. [Farsi]
Type of Study: Research |
Subject:
Microbiology
Received: 2022/10/16 | Accepted: 2023/01/29 | Published: 2023/02/21
Received: 2022/10/16 | Accepted: 2023/01/29 | Published: 2023/02/21
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