Background and Objective: In recent years, it is
recommended to use natural materials like plant extracts and essences instead
of chemical preservatives in food industry. The main objective of this study was to evaluate the
antimicrobial activity of shoot extract of purple coneflower against some gram positive and
gram negative bacteria.
Materials and Methods: This Laboratory study
was conducted in 2013. Two extraction methods (maceration and
microwave-assisted) with three solvents (water, methanol 80% and acetone) were
used to extract the phenolic compounds from purple coneflower
shoot The micro-organisms investigated in this study were: Bacillus cereus, Listeria monocytogenes, Escherichia
coli, Shigella dysenteriae and Pseudomonas
aeruginosa. Experimental
data were analyzed using ANOVA by the SPSS version 15 software and mean
comparison were done using the Duncan's multiple range test.
Results: The highest total phenolic
content was related to the methanol extract produced by microwave-assisted
extraction (MAE), whereas in both extraction methods, the lowest amount of
phenolic was obtained by using acetone extract. Comparing the extraction
methods showed that MAE had higher extraction efficiency in all three tested
solvents. Regarding to antimicrobial activity of purple coneflower shoot extracts, the minimum inhibitory concentration and minimum
bactericidal concentration were observed in gram positive bacteria (Bacillus cereus and Listeria monocytogenes). Pseudomonas aeruginosa was the most
resistant bacterium against the extracts.
Conclusion: The results showed that in most cases, the
extracts obtained by MAE method had more antimicrobial activity in comparison
to traditional method. In addition the microwave-assisted
extract of this plant can be considered as
a food preservative. However, more studies, such as
examinations in food models are needed to unravel the antimicrobial effects of given
plant.
Key words: Purple coneflower extract, Antimicrobial properties,
Extraction method.
Funding:
This research
was funded by Hamadan University of Medical Sciences and Tarbiat Modares
University.
Conflict
interest: None
declared.
Ethical
approval: This article
does not need
permission from
the Ethics
Committee because the information in this article was derived from a non-animal research.
How to cite this article: Izadi
Z, Sorooshzadeh A, Modarres Sanavi S.A.M, Esna-Ashari M, AghaAlikhani M,
Davoodi P. Effect of Extraction Method on Antimicrobial Properties
of Shoot Extract of Purple Coneflower (Echinacea Purpurea l.) Against Some Pathogenic
Bacteria?. J
Rafsanjan Univ Med Sci 2014
13(3): 267-80.
[Farsi]
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