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Background and
Objective: Angiogenesis- formation of
new blood vessels from former ones- is an essential process
in development. Bee venom has been used to cure diverse
ills for many years. In the present research the inhibitory effect of bee venom on angiogenesis has been investigated in a wistar
rat aortic model.
Materials and Methods: Rat's aorta
was cut into 1 mm pieces and cultured in collagen matrix.
After observation of the first sprouting of angiogenesis, the samples were
divided into control and experimental groups. Experimental groups were treated
with bee venom in concentration 10, 20, 30, 40,
50, 60, 70 µg/ml. Angiogenesis
was investigated and photographed by invert microscope. Numbers and lengths of
vessels were measured by Image J software. Data were analyzed by SPSS software
and ANOVA & TUKEY in significant level (p<0.05).
Results:
Lengths average and
numbers of blood vessels in experimental groups
at concentration of 10, 20, 30µg/ml in comparison to control group showed any
significant changes (p>0.05).
length average and numbers of blood
vessels in experimental groups
with 40, 50, 60, 70 µg/ml concentration showed a significant decrease in comparison to
control group )p<0.05).
Conclusion: The proper concentrations
of BV inhibits angiogenesis. So it can be considered in studies of cancer
and pathological
conditions associated
with angiogenesis.
Key
words: Angiogenesis,
Venom, Aorta, Cancer, Bee
Funding: This research was funded by
Islamic Azad University of Mashhad.
Conflict
of Interest:
None declared.
Ethical
approval: The
Ethics Committee of Islamic Azad University, Mashhad Branch, approved the study.
How to cite this article: Baharara J, Hosseini N, Ramezani T. Angiogenesis Inhibition by Bee
Venom in Rat Aortic Model. J RafsanjanUniv Med Sci 2014 13(4): 361-70. [Farsi]
Received: 2013/12/24 | Accepted: 2014/10/27 | Published: 2014/10/27
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