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URL: http://journal.rums.ac.ir/article-1-2196-en.html
Background
and Objective: Staphylococcus
epidermidis produces extracellular polysaccharide which is known as a biofilm.
Biofilm is highly effective in establishing of this bacterium infections and
can be formed on medical devices that are used in the body. The purpose of this
study was to evaluate the effects of silver colloidal nanoparticles on
bacterial growth and biofilm formation of Staphylococcus epidermidis.
Materials
and Methods: In
this empirical and cross-sectional study, experiments were performed on 13
clinical isolated specimens of Staphylococcus epidermidis and a
reference strain (ATCC 12228). The minimum inhibitory concentration
(MIC) and the minimum bactericidal concentration (MBC) of nanoparticles were
determined by the agar dilution method. The experiments of biofilm formation
were done by micro titer plate method and stained by Safranin. Data were
analyzed using ANOVA.
Results:
The mean MIC and
MBC of nanoparticles for clinical isolated specimens were 350 ± 19.61 ppm and
707.69 ± 64.05 ppm, respectively and nanoparticles had bacteriostatic
effect at low concentrations . Nanoparticles showed the anti-biofilm activity
at the concentration of 0.5 ppm and this effect enhanced with increasing
concentration up to 4 ppm. Nanoparticles at higher concentrations of 150 ppm
had the ability to eliminate the pre-formed biofilm.
Conclusion: The results showed that
colloidal silver nanoparticles have the ability to inhibit the S.
epidermidis biofilm formation. It is offered to use these compounds to coat
prosthetic devices which are placed in the body or to cover environmental surfaces
in medical centers.
Key
words: Staphylococcus
epidermidis,
Biofilm, Micro titer plate
Funding:
This research
was funded personally.
Conflict of interest: None declared.
Ethical
approval: This
research was conducted in accordance with ethical principles on clinical
specimens.
How to cite this article: Mortazavi
H, Nakhaei Moghaddam M, Nejad Shahrokhabadi Kh. Study of the Effect of Silver Nano particles on Biofilms Formation by Staphylococcus Epidermidis. J RafsanjanUniv Med Sci 2015 14(2): 125-36. [Farsi]
Background
and Objective: Staphylococcus
epidermidis produces extracellular polysaccharide which is known as a biofilm.
Biofilm is highly effective in establishing of this bacterium infections and
can be formed on medical devices that are used in the body. The purpose of this
study was to evaluate the effects of silver colloidal nanoparticles on
bacterial growth and biofilm formation of Staphylococcus epidermidis.
Materials
and Methods: In
this empirical and cross-sectional study, experiments were performed on 13
clinical isolated specimens of Staphylococcus epidermidis and a
reference strain (ATCC 12228). The minimum inhibitory concentration
(MIC) and the minimum bactericidal concentration (MBC) of nanoparticles were
determined by the agar dilution method. The experiments of biofilm formation
were done by micro titer plate method and stained by Safranin. Data were
analyzed using ANOVA.
Results:
The mean MIC and
MBC of nanoparticles for clinical isolated specimens were 350 ± 19.61 ppm and
707.69 ± 64.05 ppm, respectively and nanoparticles had bacteriostatic
effect at low concentrations . Nanoparticles showed the anti-biofilm activity
at the concentration of 0.5 ppm and this effect enhanced with increasing
concentration up to 4 ppm. Nanoparticles at higher concentrations of 150 ppm
had the ability to eliminate the pre-formed biofilm.
Conclusion: The results showed that
colloidal silver nanoparticles have the ability to inhibit the S.
epidermidis biofilm formation. It is offered to use these compounds to coat
prosthetic devices which are placed in the body or to cover environmental surfaces
in medical centers.
Key
words: Staphylococcus
epidermidis,
Biofilm, Micro titer plate
Funding:
This research
was funded personally.
Conflict of interest: None declared.
Ethical
approval: This
research was conducted in accordance with ethical principles on clinical
specimens.
How to cite this article: Mortazavi
H, Nakhaei Moghaddam M, Nejad Shahrokhabadi Kh. Study of the Effect of Silver Nano particles on Biofilms Formation by Staphylococcus Epidermidis. J RafsanjanUniv Med Sci 2015 14(2): 125-36. [Farsi]
Background
and Objective: Staphylococcus
epidermidis produces extracellular polysaccharide which is known as a biofilm.
Biofilm is highly effective in establishing of this bacterium infections and
can be formed on medical devices that are used in the body. The purpose of this
study was to evaluate the effects of silver colloidal nanoparticles on
bacterial growth and biofilm formation of Staphylococcus epidermidis. Materials
and Methods: In
this empirical and cross-sectional study, experiments were performed on 13
clinical isolated specimens of Staphylococcus epidermidis and a
reference strain (ATCC 12228). The minimum inhibitory concentration
(MIC) and the minimum bactericidal concentration (MBC) of nanoparticles were
determined by the agar dilution method. The experiments of biofilm formation
were done by micro titer plate method and stained by Safranin. Data were
analyzed using ANOVA. Results:
The mean MIC and
MBC of nanoparticles for clinical isolated specimens were 350 ± 19.61 ppm and
707.69 ± 64.05 ppm, respectively and nanoparticles had bacteriostatic
effect at low concentrations . Nanoparticles showed the anti-biofilm activity
at the concentration of 0.5 ppm and this effect enhanced with increasing
concentration up to 4 ppm. Nanoparticles at higher concentrations of 150 ppm
had the ability to eliminate the pre-formed biofilm. Conclusion: The results showed that
colloidal silver nanoparticles have the ability to inhibit the S.
epidermidis biofilm formation. It is offered to use these compounds to coat
prosthetic devices which are placed in the body or to cover environmental surfaces
in medical centers. Key
words: Staphylococcus
epidermidis,
Biofilm, Micro titer plate Funding:
This research
was funded personally. Conflict of interest: None declared. Ethical
approval: This
research was conducted in accordance with ethical principles on clinical
specimens. How to cite this article: Mortazavi
H, Nakhaei Moghaddam M, Nejad Shahrokhabadi Kh. Study of the Effect of Silver Nano particles on Biofilms Formation by Staphylococcus Epidermidis. J RafsanjanUniv Med Sci 2015 14(2): 125-36. [Farsi]
Received: 2014/06/12 | Accepted: 2015/04/8 | Published: 2015/05/12
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