Journal of Rafsanjan University of Medical Sciences

Background and Objective: Due to their anti-oxidant and anti-inflammatory compounds, medicinal plants can protect the liver against hepatotoxicant induced injuries. The aim of this study was to evaluate the protective effects of Allium Saralicum R.M. Fritsch (ASRMF) on CCl₄- induced hepatotoxicity in mice.

Materials and Methods: In the present experimental study, 35 male mice were divided into 5 equal groups. The negative control received 1ml/kg of olive oil and positive controls received 1 ml/kg of 50%-50% solution of olive oil and CCl₄, intraperitoneally. Three treatment groups received 200, 800, and 1600 µg/kg of ethanolic extract of ASRMF in addition to CCl₄ through gavage. All administrations were done twice a week for 45 days. On the last day, serum levels of samples of ALT (Alanine aminotransferase), AST (Aspartate aminotransferase), and ALP (Alkaline-phosphatase) enzymes were measured. Total volume of the liver, hepatocytes, sinusoids, portal veins, central veins, hepatic arteries, and bile ducts were estimated in histological sections. The data were analyzed using one-way ANOVA and post-hoc Tukeys’ tests.

Results: As compared to the CCl₄ - treated group, ethanolic extract of ASRMF could significantly decrease the rised levels of liver and also volume of the liver, hepatocytes, and sinusoids (P<0.05).

Conclusion: It seems that ethanolic extract of ASRMF at low and intermediate doses can protect the liver against hepatotoxicants induced injuries.

Key words: Allium Saralicum R.M. Fritsch, CCl₄, Mice, Hepatotoxicity, Antioxidant

Funding: There was no fund for this study.

Conflict of interest: None declared.

Ethical approval: The Ethics Committee of Razi University approved the study.

How to cite this article: Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R. Protective Effects of Ethanolic Extract of Allium Saralicum R.M. Fritsch on CCl₄- Induced Hepatotoxicity in Mice. J Rafsanjan Univ Med Sci 2017; 16(3): 227-38. [Farsi]