Volume 8, Issue 3 (12-2009)
JRUMS 2009, 8(3): 203-214 |
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Jalalpoor S, Kasra Kermanshahi R, Noohi A, Zarkesh Isfahani H, Abusaidi H. مقایسه فراوانی وجود آنزیم β-لاکتاماز در باکتریهای جداسازی شده از عفونتهای بیمارستانی. JRUMS 2009; 8 (3) :203-214
URL: http://journal.rums.ac.ir/article-1-5355-en.html
URL: http://journal.rums.ac.ir/article-1-5355-en.html
Abstract: (1433 Views)
Background and Objectives: β-lactame antibiotics are very important in the cure of diseases. b-lactamase as a virulence agent causes resistance to these antibiotics. Existence of β-lactamase in the pathogen bacteria can cause delay in the treatment. The aim of this research was to compare the frequency of β-lactamase enzyme existence in different types of isolated nosocomial infection bacteria in Alzahra hospital, Isfahan, Iran.
Materials and Methods: This laboratory study was performed in Alzahra hospital of Isfahan in 2005-2006. According to sample size estimation, 100 infection samples (blood, urine, skin) were randomly selected. Identification of bacteria was performed using microbiological methods; such as: staining, chemical test, and use of differential and selective media. To determine β–lactamase production in bacteria, acidimetric method was used. Data was analyzed using descriptive statistics.
Results: From the 100 isolated pathogen bacterias, 68.45% produced β-lactamase; 83.33% produced Staphylococcus spp. 70.95% produced Enterobacteriaceae spp. and 18.8% produced Pseudomonas 18.8%.
Conclusion: Based on the results, the frequncy of β–lactamase Enzyme existence in isolated bacteria of nosocomial infection was very high, which can be due to the increase of resistance of β–lactam antibiotics in pathogen Bacteria.
Key words: b–lactamase, β–lactame Antibiotics, Antibiotic Resistance, Pathogen Bacteria, Nosocomial Infections
Funding: This research was Funded by Isfahan University.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Isfahan University approved the study.
Materials and Methods: This laboratory study was performed in Alzahra hospital of Isfahan in 2005-2006. According to sample size estimation, 100 infection samples (blood, urine, skin) were randomly selected. Identification of bacteria was performed using microbiological methods; such as: staining, chemical test, and use of differential and selective media. To determine β–lactamase production in bacteria, acidimetric method was used. Data was analyzed using descriptive statistics.
Results: From the 100 isolated pathogen bacterias, 68.45% produced β-lactamase; 83.33% produced Staphylococcus spp. 70.95% produced Enterobacteriaceae spp. and 18.8% produced Pseudomonas 18.8%.
Conclusion: Based on the results, the frequncy of β–lactamase Enzyme existence in isolated bacteria of nosocomial infection was very high, which can be due to the increase of resistance of β–lactam antibiotics in pathogen Bacteria.
Key words: b–lactamase, β–lactame Antibiotics, Antibiotic Resistance, Pathogen Bacteria, Nosocomial Infections
Funding: This research was Funded by Isfahan University.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Isfahan University approved the study.
Keywords: –lactamase, β–lactame Antibiotics, Antibiotic Resistance, Pathogen Bacteria, Nosocomial Infections
Type of Study: Research |
Subject:
زيست شناسي
Received: 2020/05/16 | Accepted: 2020/05/16 | Published: 2020/05/16
Received: 2020/05/16 | Accepted: 2020/05/16 | Published: 2020/05/16
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