Volume 22, Issue 9 (12-2023)
JRUMS 2023, 22(9): 947-962 |
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Ethics code: IR.AUSMT.REC.1400.03
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Masoudi M, Azizi H, Gholami D, Khaki A. Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry and Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Cells of Mouse Testis: A Laboratory Study. JRUMS 2023; 22 (9) :947-962
URL: http://journal.rums.ac.ir/article-1-7042-en.html
URL: http://journal.rums.ac.ir/article-1-7042-en.html
Amol University of Special Modern Technologies, Amol, Iran
Abstract: (1088 Views)
Background and Objectives: Spermatogonial stem cells (SSCs) can generate embryonic stem-like (ES-Like) cells, both of which possess the potential for pluripotent stem cells. The gene ZBTB16 (Zinc Finger and BTB Domain Containing 16) plays various roles in signaling pathways, cellular differentiation regulation, and growth. The aim of this research was to identify signaling pathways associated with the ZBTB16 gene using bioinformatics methods and investigate the expression levels of this gene in SSCs and ES-Like cells.
Materials and Methods: In this experimental study, initially, 100 genes with connections to ZBTB16 were extracted from the protein database and analyzed using relevant software. Subsequently, ES-Like cells were extracted from mouse testicular SSCs and cultured. Then, the expression of ZBTB16 in ES-Like cells and SSCs was examined through immunocytochemistry and immunohistochemistry staining.
Results: The results indicated that the expression of ZBTB16 was demonstrated in the undifferentiated population of SSCs and ES-Like cells. It was found that deleting ZBTB16 from the gene network could disrupt undesirable signaling pathways since ZBTB16 is involved in enriching specific pathways as a specific transcription factor.
Conclusion: The experimental findings can support the analysis of spermatogonial cell growth in both in vivo and in vitro. Bioinformatics methods, by identifying biological functions that ZBTB16 regulates, have shown that ZBTB16 can be used as a target gene to weaken certain undesirable pathways.
Key words: Spermatogonial stem cells, Embryonic stem-like cells, Signaling pathways, Protein-protein interaction, ZBTB16
Funding: This study was funded by Amol University of Modern Technologies.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Amol University of Special Modern Technologies approved the study (IR.AUSMT.REC.1400.03).
How to cite this article: Masoudi Mahla, Azizi Hossein, Gholami Dariush, Khaki Amir. Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry and Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Cells of Mouse Testis: A Laboratory Study. J Rafsanjan Univ Med Sci 2023; 22 (9): 947-62. [Farsi]
Materials and Methods: In this experimental study, initially, 100 genes with connections to ZBTB16 were extracted from the protein database and analyzed using relevant software. Subsequently, ES-Like cells were extracted from mouse testicular SSCs and cultured. Then, the expression of ZBTB16 in ES-Like cells and SSCs was examined through immunocytochemistry and immunohistochemistry staining.
Results: The results indicated that the expression of ZBTB16 was demonstrated in the undifferentiated population of SSCs and ES-Like cells. It was found that deleting ZBTB16 from the gene network could disrupt undesirable signaling pathways since ZBTB16 is involved in enriching specific pathways as a specific transcription factor.
Conclusion: The experimental findings can support the analysis of spermatogonial cell growth in both in vivo and in vitro. Bioinformatics methods, by identifying biological functions that ZBTB16 regulates, have shown that ZBTB16 can be used as a target gene to weaken certain undesirable pathways.
Key words: Spermatogonial stem cells, Embryonic stem-like cells, Signaling pathways, Protein-protein interaction, ZBTB16
Funding: This study was funded by Amol University of Modern Technologies.
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of Amol University of Special Modern Technologies approved the study (IR.AUSMT.REC.1400.03).
How to cite this article: Masoudi Mahla, Azizi Hossein, Gholami Dariush, Khaki Amir. Investigation of ZBTB16 Gene Expression by Bioinformatics Analysis and Immunocytochemistry and Immunohistochemistry Methods in Embryonic Cells Derived From Spermatogonial Stem Cells of Mouse Testis: A Laboratory Study. J Rafsanjan Univ Med Sci 2023; 22 (9): 947-62. [Farsi]
Keywords: Spermatogonial stem cells, Embryonic stem-like cells, Signaling pathways, Protein-protein interaction, ZBTB16
Type of Study: Research |
Subject:
زيست شناسي
Received: 2023/06/29 | Accepted: 2023/11/18 | Published: 2023/12/19
Received: 2023/06/29 | Accepted: 2023/11/18 | Published: 2023/12/19
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