Volume 24, Issue 9 (12-2025)
JRUMS 2025, 24(9): 775-778 |
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Ethics code: IR.BUMS.REC.1398.412
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Taleb Rouhi M, Afshar Ghoochani M, Sanjarani A, Habibollahi M H. The Effect of Curcumin Nanoliposomes on Skin Wound Healing in BALB/c Mice: An Experimental Study. JRUMS 2025; 24 (9) :775-778
URL: http://journal.rums.ac.ir/article-1-7767-en.html
URL: http://journal.rums.ac.ir/article-1-7767-en.html
Abstract: (33 Views)
Background and Objectives: Wound healing is a complex process involving hemostasis, inflammation, proliferation, and tissue remodeling. Curcumin is known as a natural compound with anti-inflammatory and antioxidant properties, but its low bioavailability has limited its topical application. This study aimed to determine the effect of curcumin nanoliposomes on the healing process of skin wounds in male BALB/c mice.
Materials and Methods: in this experimental study, 48 male BALB/c mice (2.5 months old) were randomly assigned to four groups: experimental (treated with curcumin nanoliposome cream), positive control (nitrofurazone cream), negative control (placebo cream), and sham (no treatment). After wound induction on the dorsal region, treatments were topically applied. Tissue sampling was collected on days 4, 7, 10, and 14, and histological evaluations were performed using H&E and Masson’s trichrome staining. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test.
Results: The experimental group showed a significant reduction in inflammatory cells on days 4, 7, and 10 compared to the negative control and sham groups (p<0.001). This group also showed increased fibroblasts, collagen fiber synthesis, granulation tissue formation, and epithelial regeneration compared to the negative control and sham groups (p<0.001). Healing in the experimental group was comparable to, and in some parameters slightly better than, the positive control group.
Conclusion: The current study indicated that curcumin nanoliposomes can effectively promote wound healing in BALB/c mice by reducing inflammation and stimulating the proliferative phase.
Keywords: Nanoliposome, Curcumin, Wound healing, BALB/c mice
Funding: This study was funded by Birjand University of Medical Sciences.
Conflict of interest: None declared.
Ethical considerations: The Ethics Committee of Birjand University of Medical Sciences approved the study (IR.BUMS.REC.1398.412).
Authors’ contributions:
- Conceptualization: Maryam Taleb Rouhi, Mohammad Afshar Ghoochani
- Methodology: Maryam Taleb Rouhi, Mohammad Afshar Ghoochani
- Data collection: Maryam Taleb Rouhi, Ali Sanjarani
- Formal analysis: Mohammad Afshar Ghoochani, Mohammad Hossein Habibollahi
- Supervision: Mohammad Hossein Habibollahi
- Project administration: Mohammad Afshar Ghoochani, Mohammad Hossein Habibollahi
- Writing – original draft: Maryam Taleb Rouhi, Ali Sanjarani
- Writing – review & editing: Mohammad Hossein Habibollahi
Materials and Methods: in this experimental study, 48 male BALB/c mice (2.5 months old) were randomly assigned to four groups: experimental (treated with curcumin nanoliposome cream), positive control (nitrofurazone cream), negative control (placebo cream), and sham (no treatment). After wound induction on the dorsal region, treatments were topically applied. Tissue sampling was collected on days 4, 7, 10, and 14, and histological evaluations were performed using H&E and Masson’s trichrome staining. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test.
Results: The experimental group showed a significant reduction in inflammatory cells on days 4, 7, and 10 compared to the negative control and sham groups (p<0.001). This group also showed increased fibroblasts, collagen fiber synthesis, granulation tissue formation, and epithelial regeneration compared to the negative control and sham groups (p<0.001). Healing in the experimental group was comparable to, and in some parameters slightly better than, the positive control group.
Conclusion: The current study indicated that curcumin nanoliposomes can effectively promote wound healing in BALB/c mice by reducing inflammation and stimulating the proliferative phase.
Keywords: Nanoliposome, Curcumin, Wound healing, BALB/c mice
Funding: This study was funded by Birjand University of Medical Sciences.
Conflict of interest: None declared.
Ethical considerations: The Ethics Committee of Birjand University of Medical Sciences approved the study (IR.BUMS.REC.1398.412).
Authors’ contributions:
- Conceptualization: Maryam Taleb Rouhi, Mohammad Afshar Ghoochani
- Methodology: Maryam Taleb Rouhi, Mohammad Afshar Ghoochani
- Data collection: Maryam Taleb Rouhi, Ali Sanjarani
- Formal analysis: Mohammad Afshar Ghoochani, Mohammad Hossein Habibollahi
- Supervision: Mohammad Hossein Habibollahi
- Project administration: Mohammad Afshar Ghoochani, Mohammad Hossein Habibollahi
- Writing – original draft: Maryam Taleb Rouhi, Ali Sanjarani
- Writing – review & editing: Mohammad Hossein Habibollahi
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Citation: Taleb Rouhi M, Afshar Ghoochani M, Sanjarani A, Habibollahi MH. The Effect of Curcumin Nanoliposomes on Skin Wound Healing in BALB/c Mice: A Experimental Study. J Rafsanjan Univ Med Sci 2025; 24 (9): 775-88. [Farsi]
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Type of Study: Research |
Subject:
علوم تشريح
Received: 2025/06/23 | Accepted: 2025/11/17 | Published: 2025/12/19
Received: 2025/06/23 | Accepted: 2025/11/17 | Published: 2025/12/19
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