Journal of Rafsanjan University of Medical Sciences

  The Effect of Unilateral Testicular Blunt Trauma on Number and Motility of Sperms in Contra Lateral Testis of Wistar Rat

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  Gh. Saki [1] , K. Radan [2] , Sh. Mostafa Radmard², I. Rashidi [3]

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  Received: 27/10/09 Sent for Revision: 16/02/10 Received Revised Manuscript: 13/06/10 Accepted: 20/06/10

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  Background and Objectives: There are conflicting reports about the effects of blunt trauma on fertility. This study was designed to evaluate the effect of unilateral blunt testicular trauma on sperm parameter of contalateral testis and the protective effect of zinc on possible damage.

  Materials and Methods: This experimental study was conducted in the cell culture laboratory, Department of Anatomy of Ahvaz Jundishapur University of Medical Sciences, in 2006. T hirty prepubertal male wistar rats were divided randomly into three equal groups. Group1 was used as control. Rats in group II and group III, as blunt testicular trauma groups, were subjected to left blunt testicular trauma. After the trauma, animals in group III were given 30 mg/kg zinc sulfate intraperitoneally and this treatment was continued for 40 days at a dose of 500 ppm per day orally. All the right epididymis of rats were removed and the number and motility of sperms were examined using Makler chamber and inverted microscope.

  Results: The number of sperms in group II was significantly decreased compared to the other two groups (p<0.05). Our results showed that groups II and III have significantly lower sperm motility in comparisons with group I (p<0.05). However there was not any significant differences in sperm mobility between group II and III.

  Conclusion: These results suggested that unilateral blunt testis trauma has a negative effect on sperm count and progressive motility of sperm. Zinc has a protective effect on sperms' count, but has no protective effect on motility of sperms.

  Key words: Trauma, Sperm count, Sperm Motility, Zinc, Rat

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  Funding : This research was funded by Ahvaz Jundishapur University of Medical Sciences.

  Conflict of interest: None declared.

  Ethical approval : The Ethics Committee of Ahvaz Jundishapur University of Medical Sciences approved the study.

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  [1] - Associate Prof., Physiology Research Center, Jundishapur University of Medical Sciences, Ahvaz , Iran

  (Corresponding Author) Tel: (0611 ) 3330074, Fax: (0611) 3332036, E-mail: ghasemsaki@yahoo.com .

  [2] - Assistant Prof., Dept. of Urology , Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran

  [3] - Associate Prof., Dept. of Pathology, Faculty of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran