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Angiogenesis Inhibition by Bee Venom in Rat Aortic Model
J. Baharara[1], N. Hosseini[2], T. Ramezani[3]
Received: 21/01/2014 Sent for Revision: 04/03/2014 Received Revised Manuscript: 10/06/2014 Accepted: 22/06/2014
Background and Objective: Angiogenesis- formation of new blood vessels from former ones- is an essential process in development. Bee venom has been used to cure diverse ills for many years. In the present research the inhibitory effect of bee venom on angiogenesis has been investigated in a wistar rat aortic model.
Materials and Methods: Rat's aorta was cut into 1 mm pieces and cultured in collagen matrix. After observation of the first sprouting of angiogenesis, the samples were divided into control and experimental groups. Experimental groups were treated with bee venom in concentration 10, 20, 30, 40, 50, 60, 70 µg/ml. Angiogenesis was investigated and photographed by invert microscope. Numbers and lengths of vessels were measured by Image J software. Data were analyzed by SPSS software and ANOVA & TUKEY in significant level (p<0.05).
Results: Lengths average and numbers of blood vessels in experimental groups at concentration of 10, 20, 30µg/ml in comparison to control group showed any significant changes (p>0.05). length average and numbers of blood vessels in experimental groups with 40, 50, 60, 70 µg/ml concentration showed a significant decrease in comparison to control group )p<0.05).
Conclusion: The proper concentrations of BV inhibits angiogenesis. So it can be considered in studies of cancer and pathological conditions associated with angiogenesis.
Key words: Angiogenesis, Venom, Aorta, Cancer, Bee
Funding: This research was funded by Islamic Azad University of Mashhad.
Conflict of Interest: None declared.
Ethical approval: The Ethics Committee of Islamic Azad University, Mashhad Branch, approved the study.
How to cite this article: Baharara J, Hosseini N, Ramezani T. Angiogenesis Inhibition by Bee Venom in Rat Aortic Model. J RafsanjanUniv Med Sci 2014; 13(4): 361-70. [Farsi]
[1]- Associate Prof., Dept. of Animal Developmental Biology, Research Center for Animal Development Applied Biology & Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
(Corresponding Author): Tel: (0511) 8437092, Fax: (0511) 8437092, E-mail: baharara@yahoo.com
[2]- MSc Student in Cell & Developmental Biology, Department Biology,Faculty of Sciences, Young Researchers Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
[3]- Ph.D Student, Animal Developmental BiologyFaculty of, Biological Sciences, Kharazmi University, Tehran, Iran
Received: 2013/12/24 | Accepted: 2014/10/27 | Published: 2014/10/27
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