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JRUMS 2020, 19(6): 619-632 |
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Daneshyar S, Pouyandeh Ravan A, Khosravi A, Fourotan Y. The Long-Term Effect of High Fat Diet and Regular Aerobic Exercise Training on Gene Expression of Isoforms of Mitochondrial Creatine Kinase (Ckmt1,2) in White Adipose Tissue of Mice: An Experimental Study. JRUMS 2020; 19 (6) :619-632
URL: http://journal.rums.ac.ir/article-1-5258-en.html
URL: http://journal.rums.ac.ir/article-1-5258-en.html
Ayatollah Alozma Boroujerdi University
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The Long-Term Effect of High Fat Diet and Regular Aerobic Exercise Training on Gene Expression of Isoforms of Mitochondrial Creatine Kinase (Ckmt1,2) in White Adipose Tissue of Mice: An Experimental Study
S. Daneshyar[1], A. Pouyandeh Ravan[2], A. Khosravi[3], Y. Foroutan[4]
Received: 22/04/2020 Sent for Revision: 16/06/2020 Received Revised Manuscript: 12/07/2020 Accepted: 19/07/2020
Background and Objectives: Mitochondrial creatine kinase involves in UCP-independent thermogenesis. It isthought that the agent can be affected by nutrition and exercise. The aim of this study was to investigate the long-term effect of high fat diet and regular aerobic exercise training on gene expression of mitochondrial creatine kinase 1 (CKmt1) and mitochondrial creatine kinase 2 (CKmt2) in white adipose tissue of mice.
Materials and Methods: In this experimental study, 28 male C57BL/6 mice were divide into four groups including control (n=7), high fat diet (HFD) (n=7), exercise training (ET) (n=7) and HFD-exercise training (HFD-ET) (n=7). The subjects of the HFD groups were fed a high-fat diet (fat: %40) for a period of 12 weeks. The subjects of the training groups underwent continuous training for six weeks. The Real Time–PCR method was used to measure the expression levels of the Ckmt1and Ckmt2 genes. The two-way ANOVA was applied to analyze data.
Results: Data showed that the HFD (p=0.324) and ET (p=0.136) did not significantly affect the gene expression level of CKmt1. However, the gene expression level of CKmt2 was significantly decreased and increased by HFD and ET versus high fat diet group, respectively (p=0.043; p=0.001, respectively).
Conclusion: The findings of this study indicated that the long term feeding of high fat diet and regular aerobic training could probably affect the non-shivering thermogenesis at white adipose tissue by decreasing and increasing the expression of mitochondrial creatine kinase 2 (CKmt2), respectively.
Key words: Obesity, High fat diet, Thermogenesis, White adipose tissue, C57BL/6 mice
Funding: This study was partly funded by Ayatollah Alozma Boroujerdi University as research project (15664-13729).
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of the Ayatollah Alozma Boroujerdi University has approved the study [11] design (ABRU.AC.IR/15664-96.43).
How to cite this article: Daneshyar S, Pouyandeh Ravan A, Khosravi A, Foroutan Y. The Long-Term Effect of High Fat Diet and Regular Aerobic Exercise Training on Gene Expression of Isoforms of Mitochondrial Creatine Kinase (Ckmt1,2) in White Adipose Tissue of Mice: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (6): 619-32. [Farsi]
[1]- Assistant Prof. of Exercise Physiology, Dept., of Physical Education, Faculty of Humanities, Ayatollah Alozma Boroujerdi University, Lorestan, Iran, ORCID: 0000-0002-8806-9736
(Corresponding Author) Tel: (066) 42468320, Fax: (066) 42468321, E-mail: s.daneshyar@abru.ac.ir
S. Daneshyar[1], A. Pouyandeh Ravan[2], A. Khosravi[3], Y. Foroutan[4]
Received: 22/04/2020 Sent for Revision: 16/06/2020 Received Revised Manuscript: 12/07/2020 Accepted: 19/07/2020
Background and Objectives: Mitochondrial creatine kinase involves in UCP-independent thermogenesis. It isthought that the agent can be affected by nutrition and exercise. The aim of this study was to investigate the long-term effect of high fat diet and regular aerobic exercise training on gene expression of mitochondrial creatine kinase 1 (CKmt1) and mitochondrial creatine kinase 2 (CKmt2) in white adipose tissue of mice.
Materials and Methods: In this experimental study, 28 male C57BL/6 mice were divide into four groups including control (n=7), high fat diet (HFD) (n=7), exercise training (ET) (n=7) and HFD-exercise training (HFD-ET) (n=7). The subjects of the HFD groups were fed a high-fat diet (fat: %40) for a period of 12 weeks. The subjects of the training groups underwent continuous training for six weeks. The Real Time–PCR method was used to measure the expression levels of the Ckmt1and Ckmt2 genes. The two-way ANOVA was applied to analyze data.
Results: Data showed that the HFD (p=0.324) and ET (p=0.136) did not significantly affect the gene expression level of CKmt1. However, the gene expression level of CKmt2 was significantly decreased and increased by HFD and ET versus high fat diet group, respectively (p=0.043; p=0.001, respectively).
Conclusion: The findings of this study indicated that the long term feeding of high fat diet and regular aerobic training could probably affect the non-shivering thermogenesis at white adipose tissue by decreasing and increasing the expression of mitochondrial creatine kinase 2 (CKmt2), respectively.
Key words: Obesity, High fat diet, Thermogenesis, White adipose tissue, C57BL/6 mice
Funding: This study was partly funded by Ayatollah Alozma Boroujerdi University as research project (15664-13729).
Conflict of interest: None declared.
Ethical approval: The Ethics Committee of the Ayatollah Alozma Boroujerdi University has approved the study [11] design (ABRU.AC.IR/15664-96.43).
How to cite this article: Daneshyar S, Pouyandeh Ravan A, Khosravi A, Foroutan Y. The Long-Term Effect of High Fat Diet and Regular Aerobic Exercise Training on Gene Expression of Isoforms of Mitochondrial Creatine Kinase (Ckmt1,2) in White Adipose Tissue of Mice: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (6): 619-32. [Farsi]
(Corresponding Author) Tel: (066) 42468320, Fax: (066) 42468321, E-mail: s.daneshyar@abru.ac.ir
[2]- MSc in Clinical Biochemistry, Dept. of Biochemistry, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran, ORCID: 0000-0001-8080-2596
[3]- Assistant Prof. of Exercise Physiology, Dept. of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi, Lorestan, Iran, ORCID: 0000-0002-4728-8991
[4]- MSc in Exercise Physiology, Dept. of Physical Education and Sport Sciences, Islamic Azad University of Hamedan (Asadabad), Hamedan, Iran, ORCID: 0000-0003-1196-2830
Type of Study: Research |
Subject:
Physiology
Received: 2020/04/20 | Accepted: 2020/07/19 | Published: 2020/09/20
Received: 2020/04/20 | Accepted: 2020/07/19 | Published: 2020/09/20
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