Background and
Objective: Diabetes mellitus is one of the most
prevalent metabolic diseases
and causes testicular damage by rising
oxidative stress. Using antioxidants reduces oxidative stress and the
amount of testicular damage. In
this study, the
effect of garlic extract on markers of oxidative
stress in diabetic rats testes has
been studied.
Materials
and Methods: In
this experimental study, 24 male Wistar rats
were randomly divided into three groups:
Control, Diabetic and Diabetic with
garlic extract. Control and diabetic
groups received distilled water (1ml/100g BW) and diabetic with garlic
extract received garlic extract (1ml/100g BW,equivalent to 400 mg/100 g BW) for 6
weeks. Diabete was induced by a single intraperitoneal injection of streptozotocin at a dosage of 60 mg/kg BW. After 6 weeks, the
right testis removed and activity of oxidative stress enzymes including
malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) were
studied.
Results:
Treatment of diabetic rats with garlic extract significantly
reduced blood glucose levels (p<0.001). Also during diabete, the
level of MDA enzymes activity was increased and the level of SOD and CAT enzymes activity decreased in the testes. Treatment with the garlic extract significantly improved these parameters (p<0.001).
Conclusion:
The results of this study confirm the antioxidant role of garlic
extract on improving the testicular damage caused by diabete.
Key words: Oxidative
Stress, Diabetes Mellitus, Garlic Extract, Testis
Funding:
This research
was funded by Mashhad University of Medical
sciences.
Conflict
of Interest: None
declared.
Ethical approval: The
Ethics Committee of Mashhad University of Medical Sciences approved the study.
How to cite this article: Salimnejad R, Jalali M, Nikravesh
MR, Fazel AR. Effect of Garlic Aqueous Extract on Markers of Oxidative
Stress in Diabetic Rats Testes. J RafsanjanUniv Med Sci 2014 13(4): 371-82. [Farsi]
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