Background
and Objective: Salmonella
infections, or salmonellosis such as typhoid, bacteremia, enterocolitis
are the major health problem worldwide, especially in developing countries
including Iran. This study aimed to evaluate the antibacterial effect of
methanol and aqueous extracts of Vaccinium arctostaphylos fruit against Salmonella
spp.
Materials
and Methods: The
present study is an experimental one Methanol and aqueous extracts of Vaccinium
arctostaphylos fruit were prepared by maceration and Soxhlet method.
Antimicrobial effects of the extracts were evaluated by agar diffusion method
against Salmonella typhi (PTCC: 1609) and six clinical strains of Salmonella.
Minimal inhibitory concentration (MIC) and minimal bactericidal concentration
(MBC) were determined by micro-dilution method.
Results: All of the studied strains were
sensitive to the aqueous and methanol extracts of Vaccinium arctostaphylos
fruit. The mean zones of inhibition were obtained in ranging from 6.6 to 26.6
mm. Statistical analysis showed a significant difference between the mean
diameters of inhibition zone and increased extracts concentrations
(p<0.001). The MIC values were ranged from 50-200 mg/ml, whereas the MBC
values from 100- 400 mg/ml.
Conclusion: Methanol and aqueous extracts of
Vaccinium arctostaphylos fruit had inhibitory activity against Salmonella
spp.
Key words: Salmonella, Vaccinium arctostaphylos,
Methanol extract, Aqueous extract, Inhibitory effect
Funding: This research was funded by
Falavarjan Branch, Islamic Azad University.
Conflict of interest: None declared.
Ethical
approval: This
article does not need permission from the Ethics Committee because the
information in this article was derived from a non-animal research.
How to cite this article: Moeini
F, Mahammadi-Sichani M, Shahanipoor K. Evaluation of the Antibacterial Effect
of Methanol and Aqueous Extracts of Vaccinium Arctostaphylos Fruit
against Salmonella spp in vitro. J RafsanjanUniv Med Sci 2015 14(4): 257-68.
[Farsi]
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