Volume 11, Issue 4 (8-2012)
JRUMS 2012, 11(4): 355-364 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
jafari3970@yahoo.com. Skin Wound Healing of Chronic Diabetic Rats Using Omega-3 and Omega-6 Fatty Acids. JRUMS 2012; 11 (4) :355-364
URL: http://journal.rums.ac.ir/article-1-5313-en.html
URL: http://journal.rums.ac.ir/article-1-5313-en.html
Abstract: (1487 Views)
Background and Objectives: Scientists are trying to find ways for skin wound healing in chronic diabetic diseases. The potential role of omega-3(ω-3) and omega-6(ω-6) fatty acids on wound healing is of interest and controversial. In this study, the effects of topical application of fish and corn oils containing ω-3 and ω-6 fatty acids on skin wound healing in chronic diabetic rats has been evaluated.
Materials and Methods: In this intervention-experimental study, mature male rats were randomly divided into five groups (n=7). Diabetes in four groups were induced by subcutaneous injection of 50 mg/kg streptozotocin(STZ). The fifth group was served as normal or control group. In diabetic groups, one group was non- treated group (shame group), and two groups received fish and corn oil (FO-group and CO- group) respectively. The last diabetic group was treated with both fish and corn oil (FCO-group). All animals were wounded by a vertical 3 cm incision in the middle of their dorsum. Treatment was done 4 weeks after the induction of diabetes till complete wound healing. Wound surface area was measured at 3th, 7th, 11th, 15th and 20th post-operated days. At the same times, the histological characteristics were studied by using hematoxilin-eosine method. Required time for full healing was also measured.
Results: Our results showed that surface area of wound in FCO- group was lower than non- treated group at 11th, 15th, and 20th post- operative days significantly. Moreover the percentage of the wound healing in FCO- group was 98% at the 20th day, while this parameter in non-treated group was 70%. Histological studies showed that epidermal growth, cellular diffusion, density of collagen in FCO- group was approximately the same as the control group.
Conclusion: The results showed that topical application of fish and corn oil together may result in an acceleration of skin wound healing in chronic diabetic rats.
Key words: Diabetes, Fish oil, Corn Oil, Wound healing
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of Interest: None declared
Ethical approval: The Ethics Committee of Rafsanjani University of Medical Sciences approved the study.
How to cite this article: Jafari naveh H.R, Taghavi M.M, Shariati M, Rezvani A. Skin Wound Healing of Chronic Diabetic Rats Using Omega-3 and Omega-6 Fatty Acids. J Rafsanjan Univ Med Scie 2012; 11(4): 355-64. [Farsi]
Materials and Methods: In this intervention-experimental study, mature male rats were randomly divided into five groups (n=7). Diabetes in four groups were induced by subcutaneous injection of 50 mg/kg streptozotocin(STZ). The fifth group was served as normal or control group. In diabetic groups, one group was non- treated group (shame group), and two groups received fish and corn oil (FO-group and CO- group) respectively. The last diabetic group was treated with both fish and corn oil (FCO-group). All animals were wounded by a vertical 3 cm incision in the middle of their dorsum. Treatment was done 4 weeks after the induction of diabetes till complete wound healing. Wound surface area was measured at 3th, 7th, 11th, 15th and 20th post-operated days. At the same times, the histological characteristics were studied by using hematoxilin-eosine method. Required time for full healing was also measured.
Results: Our results showed that surface area of wound in FCO- group was lower than non- treated group at 11th, 15th, and 20th post- operative days significantly. Moreover the percentage of the wound healing in FCO- group was 98% at the 20th day, while this parameter in non-treated group was 70%. Histological studies showed that epidermal growth, cellular diffusion, density of collagen in FCO- group was approximately the same as the control group.
Conclusion: The results showed that topical application of fish and corn oil together may result in an acceleration of skin wound healing in chronic diabetic rats.
Key words: Diabetes, Fish oil, Corn Oil, Wound healing
Funding: This research was funded by Rafsanjan University of Medical Sciences.
Conflict of Interest: None declared
Ethical approval: The Ethics Committee of Rafsanjani University of Medical Sciences approved the study.
How to cite this article: Jafari naveh H.R, Taghavi M.M, Shariati M, Rezvani A. Skin Wound Healing of Chronic Diabetic Rats Using Omega-3 and Omega-6 Fatty Acids. J Rafsanjan Univ Med Scie 2012; 11(4): 355-64. [Farsi]
Type of Study: Research |
Subject:
Anatomy
Received: 2020/05/10 | Accepted: 2020/05/10 | Published: 2020/05/10
Received: 2020/05/10 | Accepted: 2020/05/10 | Published: 2020/05/10
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
