Journal of Rafsanjan University of Medical Sciences
Volume 25, Issue 2 (7-2026)
JRUMS 2026, 25(2): 103-116 |
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Ethics code: IR.UMZ.REC.1401.003
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Sadeghi S, Hajizadeh Moghaddam A, Khanjani Jelodar S, Hasantabar V. The Effect of Hesperetin Nanophytosome on Carrageenan-Induced Acute Inflammation in Mice: An Experimental Study. JRUMS 2026; 25 (2) :103-116
URL: http://journal.rums.ac.ir/article-1-7862-en.html
URL: http://journal.rums.ac.ir/article-1-7862-en.html
Mazandaran University
Abstract: (26 Views)
Background and Objectives: Oxidative stress plays a critical role in the progression of inflammatory responses. Phytosomal delivery systems, as lipid-based nanocarriers, offer a potent strategy to augment the antioxidant activity of polyphenolic compounds like hesperetin This study was designed and conducted to evaluate the anti-inflammatory effects of hesperetin nano-phytosome in a carrageenan-induced acute inflammation model in small laboratory mice.
Materials and Methods: In this experimental study, 35 male small laboratory mice were randomly divided into five groups (n=7): the control group received no treatment; the second group received hesperetin nano-phytosome orally at a dose of 20 mg/kg for 14 days and carrageenan vehicle via intraplantar injection; the third group received a subplantar injection of 1% carrageenan; and the fourth and fifth groups received oral hesperetin and hesperetin nano-phytosome for 14 days, followed by carrageenan injection. Inflammatory pain was evaluated using the hot plate test. At the end of the experiment, antioxidant enzyme activities, as well as glutathione and malondialdehyde levels, were measured. Data were analyzed using one-way analysis of variance followed by Tukey’s post hoc test.
Results: Hesperetin nano-phytosome significantly increased pain response latency compared with the carrageenan group. Both hesperetin and its nano-phytosome significantly reduced malondialdehyde levels and increased glutathione levels as well as the activities of catalase, superoxide dismutase, and glutathione peroxidase compared with the carrageenan-treated group (p<0.05).
Conclusion: The results of the present study indicate that hesperetin nano-phytosome is likely to exert its anti-inflammatory effects through modulation of oxidative stress.
Keywords: Inflammation, Carrageenan, Hesperetin, Mice, Nanophytosome, Oxidative stress
Funding: This study did not receive any funds.
Conflict of interest: None declared.
Ethical considerations: This study was approved by the Ethics Committee of the University of Mazandaran, Babolsar, Iran (IR.UMZ.REC.1401.003).
Authors’ contributions:
- Conceptualization: Akbar Hajizadeh Moghaddam
- Methodology: Vahid Hasantabar
- Data collection: Simin Sadeghi Mahali
- Formal analysis: Sedigheh Khanjani Jelodar
- Supervision: Akbar Hajizadeh Moghaddam
- Project administration: Sedigheh Khanjani Jelodar
- Writing – original draft: Simin Sadeghi Mahali
- Writing – review & editing: Akbar Hajizadeh Moghaddam, Sedigheh Khanjani Jelodar
Materials and Methods: In this experimental study, 35 male small laboratory mice were randomly divided into five groups (n=7): the control group received no treatment; the second group received hesperetin nano-phytosome orally at a dose of 20 mg/kg for 14 days and carrageenan vehicle via intraplantar injection; the third group received a subplantar injection of 1% carrageenan; and the fourth and fifth groups received oral hesperetin and hesperetin nano-phytosome for 14 days, followed by carrageenan injection. Inflammatory pain was evaluated using the hot plate test. At the end of the experiment, antioxidant enzyme activities, as well as glutathione and malondialdehyde levels, were measured. Data were analyzed using one-way analysis of variance followed by Tukey’s post hoc test.
Results: Hesperetin nano-phytosome significantly increased pain response latency compared with the carrageenan group. Both hesperetin and its nano-phytosome significantly reduced malondialdehyde levels and increased glutathione levels as well as the activities of catalase, superoxide dismutase, and glutathione peroxidase compared with the carrageenan-treated group (p<0.05).
Conclusion: The results of the present study indicate that hesperetin nano-phytosome is likely to exert its anti-inflammatory effects through modulation of oxidative stress.
Keywords: Inflammation, Carrageenan, Hesperetin, Mice, Nanophytosome, Oxidative stress
Funding: This study did not receive any funds.
Conflict of interest: None declared.
Ethical considerations: This study was approved by the Ethics Committee of the University of Mazandaran, Babolsar, Iran (IR.UMZ.REC.1401.003).
Authors’ contributions:
- Conceptualization: Akbar Hajizadeh Moghaddam
- Methodology: Vahid Hasantabar
- Data collection: Simin Sadeghi Mahali
- Formal analysis: Sedigheh Khanjani Jelodar
- Supervision: Akbar Hajizadeh Moghaddam
- Project administration: Sedigheh Khanjani Jelodar
- Writing – original draft: Simin Sadeghi Mahali
- Writing – review & editing: Akbar Hajizadeh Moghaddam, Sedigheh Khanjani Jelodar
Type of Study: Research |
Subject:
Physiology
Received: 2025/10/16 | Accepted: 2026/06/10 | Published: 2026/07/28
Received: 2025/10/16 | Accepted: 2026/06/10 | Published: 2026/07/28
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